Therapeutic effect of VEGF receptor inhibitor SU5614 in a murine model of toluene diisocyanate induced asthma

Abstract

Abstract: AIM: To discuss the therapeutic effect of vascular endothelial growth factor (VEGF) receptor inhibitor SU5614 in a murine model of toluene diisocynate(TDI) induced asthma.METHODS: BALB/C mice were randomly divided into three groups: the control group, the model group and the SU5614 group.Using the TDI induced asthma in mice, the effect of SU5614 was evaluated on expression of VEGF in bronchoalveolar lavage fluid(BALF),pathological changes of lung tissue,determination of airway responsiveness to methacholine and measurement of plasma exudation.RESULTS: Immune cytological analysis showed that expression of VEGF in BALF was significantly increased in model group, but significantly decreased in the SU5614 group. The airway inflammation of lung tissue was expressed obviously in model group, but significantly reduced in the SU5614 group.The airway responsiveness increased in model group, while in the SU5614 group it was significantly reduced (P<0.05).Plasma exudation was significantly increased in model group, but significantly decreased in the SU5614 group (P<0.05).CONCLUSION: SU5614 not only inhibit VEGF activity in BALF,but also inhibit the migration of inflammatory cells, reduce airway inflammation and plasma exudation.

Key words: asthma, vascular endothelial growth factor, toluene diisocyanate, vascular endothelial growth factor receptor inhibitor, SU5614

CLC Number:

R965.2

JIANG Ai-ying, YU Ren-zhi, LV Yu-feng, LUO Hai-long. Therapeutic effect of VEGF receptor inhibitor SU5614 in a murine model of toluene diisocyanate induced asthma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 269-273.

References

[1] 王雅娟,朱慧志,孔献春,等.川芎嗪改善哮喘大鼠气道高反应性的作用观察[J].中成药,2014,36(4):834-837.
[2] 沈巨信,秦娥,李明晖,等.呼出气冷凝液白三烯、8异前列腺素、硝酸盐/亚硝酸盐检测在哮喘中的应用及孟鲁司特对炎症指标的影响[J].中国临床药理学与治疗学,2012,17(7):802-805.
[3] 方泓,喻晓,吴银根,等.咳喘六味合剂对RSV感染哮喘大鼠气道炎症和气道高反应性的影响[J].上海中医药杂志,2013,47(12):66-69.
[4] 潘丰满,张德新,李祥华,等.止哮平喘方对哮喘大鼠气道高反应性的影响[J].中国老年学杂志,2013,33(17):4215-4217.
[5] 刘春丽,赖克方,陈如冲,等.迷走神经在盐酸灌注食管诱导的气道神经源性炎症中的作用[J].广东医学,2007,28(7):1027-1029.
[6] 温亚男,李涛,陈永青,等.某甲苯二异氰酸酯生产项目职业病危害因素及防护措施调查[J].工业卫生与职业病,2014,40(2):86-90.
[7] 李文嘉,赵海金,董航明,等.活性维生素D对甲苯二氰酸醋哮喘模型气道屏障功能的影响及机制探讨[C].大连:中华医学会呼吸病学年会-2013 (第十四次全国呼吸病学学术会议)论文汇编,2013:187.
[8] 王立花,樊燕蓉,李鹏,等.RNAi介导肿瘤细胞VEGF基因表达临床研究的现状[J].中华肿瘤防治杂志,2012,19(5):392-396.
[9] 李良昌,李宜丹,金东洙.血管内皮生长因子受体抑制剂对哮喘模型小鼠的抗肺部炎症作用[J].延边大学医学学报,2008,31(3):175-178.
[10]马小娟,孙湛,于文燕,等.维药神香草对哮喘小鼠气道高反应性的抑制作用[J].免疫学杂志,2013,29(8):650-658.
[11]郑燕冰,赖克方,陈如冲,等.热、酸刺激豚鼠食管诱导气道血浆渗出及其机制探讨[J].中华哮喘杂志(电子版),2009,3(6):399-403.
[12]姚红卫,吕丽丽.地塞米松对哮喘小鼠气道血管生成及血管内皮生长因子表达的影响[J].重庆医学,2012,41(36):3854-3856.
[13]Hoshino M, Nakamura Y, Hamid QA. Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma[J].J Allergy Clin Immunol, 2001,107(6):1034-1038.
[14]邓娜,赵旸,董继萍. VEGF及TGF-β1水平变化在哮喘患儿中的意义[J].实用医学杂志,2011,27(16):2971-1973.
[15]张业贵,丁艳霞,侯良芹.针药结合对慢性脑缺血大鼠大脑皮质BDNF、TrkB及VEGF表达的影响[J].中国临床药理学与治疗学,2013,18(4):376-381.
[16]胡宇敏,旷寿金. VEGF和支气管哮喘的关系[J].中外医学研究,2014,12(11):150-151.
[17]赵灵芝,卢美萍.哮喘儿童支气管肺泡灌洗液IL-17、IL-18和VEGF水平测定及意义[J].中国病理生理杂志,2011,27(3):577-580.
[18]赵伟,蒋爱华,刘祖国.角膜碱烧伤后VEGF与新生血管渗漏性的相关性实验研究[J].眼科新进展,2009,2(1):18-20.
[19]刘毅.VEGF在脓毒症毛细血管渗漏中的作用[J].国际内科学杂志,2009,36(1):34-36.