Significance of ubiquitin ligase hrd1 expressed in human gastric carcinoma and normal cells

Abstract

Abstract: AIM: To investigate the expression of ubiquitin ligase hrd1 expressed in human gastric carcinoma, and discuss its significance in the occurrence and development of gastric carcinoma.METHODS: Collect the samples of gastric carcinoma and the tissue 5 cm from the edge of the cancer, research the expressions of hrd1 protein in normal human cells and gastric carcinoma cells by using immunohistochemical SP method and Q-PCR. Then use statistical methods to analyze the relationship between hrd1 expression and clinicopathological parameters of gastric cancer.RESULTS: Expression level of hrd1 protein was significantly higher in human gastric carcinoma cells than that in normal human cells (P<0.05). The lesions which has a larger size (≥5 cm) has a higher expression of hrd1 than the lesions which has a smaller size (<5 cm, P<0.05). But not with histologic grade and lymph node metastasis (P>0.05).CONCLUSION: Ubiquitin ligase hrd1 may play a role in the occurrence and development of human gastric carcinoma.

Key words: gastric carcinoma, ubiquitin-proteasome system, ubiquitin ligase

CLC Number:

R735

PENG Jun-lu, SHI Liang-hui, LI Fan-hong, ZHAO Dun-dun. Significance of ubiquitin ligase hrd1 expressed in human gastric carcinoma and normal cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 314-317.

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