Effects of total triterpenoids of prunella vulgaris L. on acetaldehyde-stimulated hepatic stellate cell-T6 and partial mechanisms

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2015, Vol. 20 ›› Issue (4): 404-408.

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Effects of total triterpenoids of prunella vulgaris L. on acetaldehyde-stimulated hepatic stellate cell-T6 and partial mechanisms

ZHANG Sheng-peng¹, LIU Xiao-ping¹, SHEN Jie², XIE Jia-li³

¹ School of Pharmacy, Wannan Medical College, Wuhu 241002, Anhui, China;
² Department of Clinical Pharmacy, Yijishan Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui, China;
³ Department of Pharmacy, Wuhu NO.2 People's Hospital, Wuhu 241001, Anhui, China

Received:2014-07-11 Revised:2015-03-24 Published:2015-05-07

Abstract

Abstract: AIM: To investigate the effects of total triterpenoids of prunella vulgaris L.(TTP) on proliferation and apoptosis in hepatic stellate cell-T6 (HSC-T6) stimulated by acetaldehyde and its partial mechanisms.METHODS: HSC-T6 cells were stimulated by acetaldehyde and treated with different doses of TTP (10, 30, 100, 300, 1000 μg/mL), the HSC-T6 proliferation were detected by MTT, the apoptosis rate were analyzed by flowcytometry, and the expression of mRNA levels of α-SMA, ProcollagenⅠ, smad2, smad3, smad7 were detected by RT-PCR.RESULTS: As compared with the model group, TTP could inhibit HSC-T6 stimulated by acetaldehyde and induce the apoptosis. Furthermore, TTP reduced the mRNA expression of α-SMA, ProcollagenⅠ, Smad2, Smad3 and increase Smad7.CONCLUSION: TTP can inhibit the proliferation of HSC-T6 stimulated by acetaldehyde and induce the apoptosis, down-regulate the expression of α-SMA, ProcollagenⅠ, which might be associated with the inhibition of the expression of Smad2、Smad3 and the increase of Smad7

Key words: total triterpenoid of prunella vulgaris L., HSC, acetaldehyde

CLC Number:

ZHANG Sheng-peng, LIU Xiao-ping, SHEN Jie, XIE Jia-li. Effects of total triterpenoids of prunella vulgaris L. on acetaldehyde-stimulated hepatic stellate cell-T6 and partial mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(4): 404-408.

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Effects of total triterpenoids of prunella vulgaris L. on acetaldehyde-stimulated hepatic stellate cell-T6 and partial mechanisms

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