Abstract:

AIM: To analyze the correlation between HPV-DNA integration and cervical cancer development as well as the expression of cancer suppressor gene p53 and pRb, so as to provide new idea for its clinical diagnosis and treatment. METHODS: 131 patients with cervical intraepithelial neoplasias (CIN) and cervical cancer which treated with surgery were selected and divided into 4 groups according to disease degree: CINⅠ, CINⅡ, CINⅢ and cervical cancer;at the same time, 33 patients with benign tumor were selected as controls. The pathological tissues were extracted and detected HPV-DNA integration using whole genome sequencing and high-throughput virus integrated experiment. According to HPV-DNA integration results, all the patients were divided into 3 groups: multi-integration, single-integration and non-integration, and the p53 and pRb expression levels between groups were compared.RESULTS:The HPV-DNA integration rate increased according to the healthy tissue, CIN Ⅰ, CIN Ⅱ, CIN Ⅲ, cervical cancer in the order, and there was mainly multi-integration in the cervical cancer, and mainly single-integration in CIN and the controls, the difference between groups had statistical significance (P<0.05). 8 types of high-risk HPV-DNA integration, including HPV16, HPV58, HPV18, HPV35, HPV31, HPV33, HPV52 and HPV51 were found, with similar distribution trend in different disease degree. The p53 and pRb positive rate and expression level were different in different integration state, and non-integration group was higher than single-integration group, single-integration group was higher than multi-integration group, the difference between groups had statistical significance (P<0.05).CONCLUSION:HPV-DNA integration can restrain the expression of p53 and pRb and promote the development of cervical cancer, which may have a certain guiding role for the identification and prognosis evaluation of cervical cancer.

Key words: human papilloma virus, DNA integratio, cervical cancer, cancer suppressor gene