Table of Content

Volume 13 Issue 4
26 April 2008

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Computer systems and GCP - site roles and responsibilities for computer systems used in clinical trials

Dr.Teri Stokes, LI Juan, Checked by XIE Hai-tang

2008, 13(4):  361-365. 

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Many computer systems can be used in the diagnosis and care of subjects in a clinical study,in dispensing drug supplies, and in performing laboratory tests required by the study protocol. It is impotant that these systems are closely controlled and perform reliably every time they are used. Intermational regulations require that such systems be well documented. Audits a and inspections at clinical study sites under Good Clinical Practice (GCP) will check such systems to be sure that they are re liable in perfomance and that their data is trustw orthy. The Principal Investigator in a study is responsible for the quality of all computer systems used to meet the study protocol and for the quality and trustworthiness of all trial data collected either by paper or computer.

The interaction of Trans -resveratrol and its glucoside Trans peceid with drug transporters

HE Hui, CHEN Xi-jing, WANG Guang-ji

2008, 13(4):  366-372. 

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AIM: To study the two stilbenes'(trans resveratrol, TR and its glucoside trans piceid,TP) interaction with A BC transporters in cell models.METHODS: The concentrations of TR，TP， and Rhodimin123 (Rh123) in cells were measured by HPLC or fluorescence spectrophotometer. Cytotoxicity was determined with MTT. The expression of P-gp was evaluated using Westem blot. RESULTS: Pgp's inhibitor and substrate (Verapamil and Rh123) had no impact on the accumulation of the two stilbenes. Pro-benecid, the inhibitor of multidrug resistance -associated protein2 (Mrp-2)，increased accumulation of TR and TP to 1.3 and 1.6 folds. TR andTP increased the uptake of Rh123 in RBMECs. Adriamycin's (ADM) cytotoxicity was also enhanced by the two stilbenes.The expression of Pgp in K562/A02 cells wasn't changed noticeably by∞- incubating with TR or TP.The stilbenes also didn' t change the cytotoxicity of Mitoxantrone (MIT) in Human non -small cell lung cancer cell (NCIH460).CONCLUSION: TR and TP inhibited P-gp from excreting substrates. They were effluent by Mrp2，not P-gp. They didn't affet the effluent of BCRP.

Effcts of Diazepam on spontaneous motor activity and learning and memory function in mice

WANG Xu-dong, WANG Ya-li, DAI Ti-jun

2008, 13(4):  373-376. 

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AIM: To study the effects of diferent doses of Diazepam on mice spontaneous motor activity,learning and memory function. METHODS: 120 Kun-ming mice were randomly divided into 4 groups. NS Group was given by hypodermic injection normnal saline with the doseof 10 mL/kg; the other 3 groups (groups Do.5，D1.o and D2.o) were given by intraperitoneal in-jection diazepam with the different doses of 0.5mg/kg,1.0mg/kg and 2.0mg/kg respectively. The mice were then conducted to take step-down test and steprthrough test to evaluate the efects of Diazepam on leaming and memory function by studying the latency of reaction and the number of errors in its perfomance. RESULTS: Compared w ith NS Group, Diazepam reduced the number of autonomic activities with dose dependently,shortened the latency of eaction and increased the number of errons of stepdown test and step-through test. CONCLUSION: Diazepam can decrease the mice spontaneous motor activity， restrain the leaming and memory function of mice，and the effects disappear quickly after 3 days.

Effects of fasudil on inflammatory reaction in cerebral ischemia /reper-fusion rats

WU Jian-hua, FANG Yun-xiang

2008, 13(4):  377-383. 

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AIM: To observe the effects of fasudil on inflammatory reaction in cerebral ischemia /reperfu-sion rats. METHODS: Focal cerebral ischemia /reper-fusion model in rats was made by transient occ lusion of middle cerebral arteryy for 1.5 h followed by 24 h reperfusion.Fasudil was injected intraperitoneally before operation and 12 h after operation. The neurological function was scored and the infarct volume was measured after operation. The water content of brain was measured with dry -wet weight; The myeloperoxidase (MPO) activity was determined by spectropho tometer;The permeability of the blood brain barrier was evaluated by measurement of the Evans Blue (EB)content in the brain with spectrophotometer; The brain content of L-8 was ev aluated with ELISA; The level of NF+κB p65 mRNA expression was detemined by RT-PCR; The expressions of ICAM-1，VCAM-1 and NF-κB p65 protein were observed by immunohistoche-mistry; The MCP-1 and the expression of NF-κB p65plotein in the nuc lear extracts were detected by westernblotting. RESULTS: Fasudil improved the neurologi-cal function, decreased the infarct size,reduced the permeability of blood brain barrier and brain water content, inhibited the MPO activity, decreased the ontent of lL-8 and the protein expressions of ICAM-1, VCAM-1 and MCP-1 in brain tissue, inhibited the expressions of NF+κB p65 mRNA and protein and reduced the content of NF-κB p65 protein in the nuclear ex tracts signifi cantly(P< 0.05 vs Model Group). CONCLUSION: These results show that the antinflammatory effect of fasudil may be correlated w ith decreasing the expression of adhesion molecu le and chemotactic factors though in hibiting the activity of NF-κB.

Determination of the AmpC and ESBL produced in the biofilm of klebsiella pneumoniae

LI Nai-jing, LI Yan, PAN Zuo-dong, HE Ping, LI Sheng-qi

2008, 13(4):  384-387. 

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AIM: To evaluate the drug fast mecha-nism of bacteria gowing in biofilm，and to provide the theoretical basis for selecting antimicrobial agents in clinic. METHODS: The model of klebsiella pneu-moniae bacterial biofilm were built up with the modifed flat-board method and identified with the method stain-ing with AgNO₃ and confocal scanning laser microscopy. The ESBLs and AmpC were detected by improved three dimensional test. RESULTS: The detection rates of AmpC，ESBLs and AmpC plus ESBLs in isolated klebsiella pneumoniae were 2.5%(1/40)，20.0%(8/40) and 2.5%(1/40),whereas the detection rates of AmpC，ESBLs and AmpC plus ESBLs in biofilm kleb-siella pneumoniae were 20.0%(840)，45.0%(18/40)and 22.5%(940). The resistance rates of the biofilm klebsiella pneumoniae producting AmpC and ESBLs to eight kinds of antibiotics were higher. CONCLUSION: The synergetic effect of the formation of biofilm and the pioduction of ESBLs and AmpC is one of the main reasons that klebsiella pneumoniae resists antibiotics.

Effects of fimbriate orostachys herb extractive on experimental gastro-helcoma animals

LI Jie, CUI Shu-xiang, ZHOU Ling, WANG Peng, MU Yan-ling

2008, 13(4):  388-391. 

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AIM: To investigate the antiulcer effects of fimbriate orostachys herb extractive on experimental gastroheloma animals.METHODS: Five kinds of animal models with experimental gastric ulcer were established and gastric mucosal injuries were induced by applying indomethac or imposing stress in mice, applying ethanol or acetic acid or pyloric ligation in rats, respectively. The rats were divided into experimental groups treated with fimbriate orostachys herb extractive 400，200，100 mg/kg, positive ontol group,and model control group. The mice were divided into experimental goups treated with fimbriate orostachys herb extractive 800, 400, 200 mg/kg, positive control group, and model group. The gastric ulcer index, gastric juice free acid output, total acid output were measured in animals. RESULTS: Administration with fimbriate orostachys herb extractive 400 and 800 mg/kgwere able to reduce the ulcer index of rats with stressu lceration(P<0.05)，and 200一800mg/kg doses of fimbriate orostachys herb extractive were able to reduce the ulcer index of mice with gastohelcoma induced by indomethac (P<0.05 or P< 0.01)and rats with gastrohelcoma induced by ethanol (P< 0.05),mean while, the gastric ulcer healing in gastrohelcoma rats accused by acetic acid was promoted obviously, but there was no significant influence on gastric secretion and pepsase. CONCLUSION: Fimbriate orostachys herb extractive has evident preventive and therapeutical effects on experimental gastrohe lcoma animals.

Effects of rhubarb on enteric mucosa structure and permeability in intestinal ischemia reperfusion rats and its mec hanisms

SUN Jia-yan, ZHU Hai-rong, TAN Ding-yu

2008, 13(4):  392-395. 

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AIM: To observe the protective effect of rhubarb on enteric mucosa strucu and permeability of rats intestine after ischemia reperfusion, and its possible mechanisms. METHODS: 30 male SD rats were divided into Rhubarb, Model and Control Groups randomly(n= 10). The model rats were established by blocking superior mesenteric artery (SMA)，and total parenteral alimentation (TPN) was done. Rhubarb and the same volume of nommal saline were given orally in Rhubarb and Model goups respectively. SMA was decoherenced but not blocked in Control Group. The 24 h urine was collected at day 3 after the operation and the enteric mucosa permeability was determined by disaccharide assay. The 10 cm jejunum and 10 cm ileum were collected at day 6 for mucosa structure and HSP70 study. RESULTS: The enteric mucosa pemmeability of Control and Rhubarb group were lower than that in Molel Group (P< 0.01)，though that in Rhubarb Group was higher than that in Contol Group, there wasno statistical diference (P> 0.05). The villi height and mucosa thickness of small intestine in Model Group were significantly lower than those of Control and Rhubarb Group(P< 0.01). Those of Rhubarb Group were similar to those of Control Group (P> 0.05). There was no statistics difference between three groups in full thickness of small intestine. The content of HSP70 in Model Group was much lower than those in Control and Rhubarb Groups(P< 0.01). The content of HSP70 in Rhubarb group was higher than that in Control Group,but there was no statistics difference (P> 0.05).CONCLUSION: Administration of rhubarb orally can decrease the enteric mucosa structure and permeability change after I/R in jury. Rhubarb protects the gut maybe by inducing more expresed HSP70 in the intestine.

Expression of Grouth- Related Oncogen-a protein in rat asthma model and effects of dex amethasone

LUO Li-dan, JIN Xiao-hong, TONG Xia-sheng, WANG En-zhi, GUO Hai-yuan, RUAN Zheng-ying, YE Hui

2008, 13(4):  396-400. 

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AIM: To observe the expressions of Grouth-Related Oncogen-a(GROa) in rat asthma model and effects of dexamethasone. METHODS: In this experiment, the rat model of asthma were randomly divided into three groups on average, incuding asthma group，control group and dexamethasone treated group.Blood PMN were isolated and purified.The expressions of GROa protein in bronchial wall and in blood PMN were detected by immunohistochemical method. Numbers of PMN were calculated in lung tissue. RESULTS: Levels of GROa protein in asthma group(0.138±0.009,Optical density) and in dexamethasone treated group(0.105±0.012, Optical density)were significant higher than those in control group(0.077±0.010，Optical density) (P<0.01) in bronchial wall. And levels of GROa protein in dexamethasone treated group were significantly decreased compared to asthma gnoup(PK 0.01). While in blood PMN，The expressions of GROa protein in asthma group(0.211土0.017, Optical density) and in dexamethasone treated goup(0.128±0.012, Optical densirty) were significant higher than those in control group(0.081±0.008，Optical density)(P<0.01). And levels of GROa protein in dexamethasone treated group were significantly decreased compared to asthma group(P<0.01). And the levels of G ROa protein in bronchial wall and blood PMN were significant different between dexamethasone treated group and those in asthma group(P<0.01). There numbers of PMN in lung tissue were significant different among three goups, in control group (7±2)< in asthma group(26±6)CONCLUSION: In bronchial wall and in blood PMN，levels of GROa protein were increased in rat asthma. And numbers of PMN in lung tissue were increased either. They are involved in inflammation of rat asthma model. The decreased inflammation of asthma by dexamethasone maybe via decreased synthesis of GROa protein. But dexamethasone can increasing PMN influxing into lung tissue. The influx of PMN into lung tissue may be corelated with GROa protein.

Protective effects of bisdemethoxycurcumin liposome on CCl₄-induced acute liver inj ury in mice and its mechanism

DAI Cheng feng, LI Juan, JIN Lu-yan

2008, 13(4):  401-405. 

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AIM: To evaluate the therapeutic effects of bisdemethoxycurcumin liposome in mice with CCL induced acute liver injury model and its mechanism. METHODS: 40 ICR mice were randomly divided into 4 groups: ontrol goup, model group, bisdemethoxycurcumin injection group and demethoxycurcumin liposome gloup (n= 10). The liver injury model was made by injecting CCl4 in mouse's abdomen. The activities of ALT, AST in serum, the contents of MDA in liver and the pathological changes of hepatic tissue were investigated. RESULTS: Compared with those in control group, the serum ALT, AST activities and liver MDA contents of model group were highly increased,but those in bisdemethoxycurcumin injection group and bisdemethoxycurcumin liposome group were reduced significantly. The liver lobules were ameliorated obviously too, especially in bisdemethoxycurcumin liposome group. The phamacodynamic action of liposome was better than that of the in jection. CONCLUSION: Bisdemethoxycurcumin liposome has significantly protective effects on CCl-induced acute chemical liver injury by reducing peroxidation of lipid in endotheliocyte of the liver.

Study on the excretion of viaminate in rats by LC-APCI-MS、MS

CAO Ling, ZHENG Feng, LIU Wen-ying, MA Peng-cheng, CHENG Xuan, SUN Di

2008, 13(4):  406-411. 

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AIM: To establish a highly sensitive, rapid and selective liquid chromatography-tandem mass spectrometry (LC-MS MS) method to determinate the viaminate in bile, urine and feces sample of rats .And to study the excretion of viaminate in rats in order to provide a guidance for the clinical trial .METHODS: The concentration of viaminate was determinated on an Aglilent TC C18 column and the mobile phase consisted of methanol-water and formic acid (93 ∶7 ∶0 .1, V/V/V) using simvastatin as internal standard .The de-tection was performed on a trip-quadrupole tandem mass spectrometer by selected ion monitoring (SRM) scan mode via atmospheric pressure chemical ionization (APCI) .The detected ions were m z 448 283 (viami-nate) and m z 419 285(internal standard) respective-ly.The accumulative excretion amount of viaminate was calculated in the urine, feces and bile, respectively.RESULTS: The extraction recoveries of viaminate in bile, urine and feces were more than 89 %.The RSD of intra-run and inter-run precisions were all less than 10 %.The calibration curves were linear over the con-centration ranges of 0 .0808 -40 .4 ng mL(r =0 .9972 and 0 .9949) for the viaminate in bile and urine .The calibration curve was linear over the concentration ranges of 0 .505 -50 .5 ng mL (r =0 .9990) for the viaminate in feces, The limit of quantitation of method for viaminate was 0 .2 pg .12 .9 %viaminate was found from feces in 72 hours, 0 .003 %was found from urine in 96 hours and 0 .0001 %was detected from bile in 24 hours.CONCLUSION: The method is proved to be accurate, sensitive, selective and convenient .It has successfully been applied to study the excretion of vi-aminate in rats .The amount of viaminate recovered from bile and urine after orally administrated to rat was very low .

Effects of a novel thiazolidinedione ANY₂ on glycometabolism in HepG₂ cells

QIAO Hai-qi, WU Guan-zhong, YANG Hong-xia

2008, 13(4):  412-417. 

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AIM: To investigate the effects of a novel thiazolidinedione ANY₂ on glycometabolism in HepG₂ cells .METHODS: HepG₂ cells are exposed to high concentrations of insulin for 24 h to build the model of insulin-resistance (IR-HepG₂) .The effects of ANY₂ , including glucose consumption (GC) , glucone-ogenesis and glucolysis were tested in this model.RESULTS: ANY₂ could dose-dependently enhance GC in IR-HepG₂ cells at high, medium and low levels of glucose.Compared with Modle Group, ANY₂ increased IR-HepG₂ cells glycogen synthesis significantly only in high dose group .It inhibited gluconeogenesis from lac-tic acid, but increased the production of lactic acid and the activity of pyruvate kinase (PK) in glucolysis.CONCLUSION: Compound ANY2 has remarkable ef-fects on improving glycometabolism in IR-HepG₂ cells.

Luteolin ameliorates endothelial dysfunction induced by oxidative stress

PAN Fang-fang, ZHOU Yong-mei, ZHANG Min, SONG Jun-na, LIU Bao-lin

2008, 13(4):  418-424. 

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AIM: To evaluate the protective effect of luteolin on endothelial dysfunction induced by tert-butyl hydro-peroxide ( t-BOOH ) .METHODS: We observed the effect of luteolin on t-BOOH-induced contractions in the aorta rings with or without endothelium, which were incubated with luteolin ( 10^-6 to 10^-4mol/L) for 30 min before determining the concentra-tion-response to t-BOOH .Cultured endothelial cell line ( ECV304) was pretreated with different concentrations of luteolin (10^-6 to 10^-4mol/L)for 30 min and hen exposed to 10^-5mol/L t-BOOH for 24 hours .Cellmorphology was observed, and cell viability was deter-mined by MTT assay .Meanwhile, RT-PCR was used to measure the expression of eNOS and COX-1 .RESULTS: Increasing concentrations of t-BOOH pro-duced concentration-dependent contractions in aorta rings isolated from rats, luteolin effectively attenuated the contraction in a concentration-dependent manner, and the relaxation response was greater in intact endo-thelium segments .In MTT and RT-PCR assays, luteo-lin effectively reduced the cytotoxicity of t-BOOH to endothelium cell and increased the expression of nitric oxide synthase ( eNOS ) mRNA, which was greatly down-regulated by t-BOOH.CONCLUSION: Luteo-lin effectively protects the endothelium from the impairment of oxidative stress, and the protection could be related to its negative modulation towards t-BOOH-in-duced contractions in the aorta .

Expression and purification of HCBP12 fusion protein and preparation of polyclonal antibody against HCBP12

LI Yue, WANG Qi, LIN Yuan, CHENG Jun, LI Kang, LI Hua

2008, 13(4):  425-430. 

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AIM: To express and purify HCV core binding protein ( HCBP12) with prokaryotic cell ex-pressive vector and to prepare HCBP12 specific rabbit polyclonal antibody .METHODS: The DNA segment of HCBP12 was amplified by RT-PCR with mRNA tem-plate from HepG2 cells and inserted into inducible pro-karyotic expressive vector pET-32a(+) .The recombi-nant plasmid, pET-32a(+)-HCBP12, was trans-formed into the competent E.coli BL21 .The inducible HCBP12 fusion protein was analyzed by SDS-PAGE and conformed by Western blot .Then the inducible HCBP12 protein w as purified and renatured by Ni⁺ affirity column chromatography. The purified HCBP12 ploteinwas used to immunize New Zea land rabbits for preparing polyclonal antibody. The specificity and potency of polyclonal antibody was evaluated by Western blot and ELISA .RESULTS: The HCBP12 fusion pro-tein was highly expressed and purified .The polyclonal antibody against HCBP12 was obtained successfully with the titer >1:512000 and confirmed specifically with Western blot .CONCLUSION: The successful ex-pression and purification of HCBP12 fusion protein and the preparation of specific anti-HCBP12 polyclonal an-tibody will be valuable for the study on the biological function of HCBP12 and clinical therapy of Hepatitis C .

Effects of estrogen on estrogen receptors in myocardium of ovariecto-mized female rats

ZHONG Xiao-hua, WANG Hui, LI Qing-ping

2008, 13(4):  431-434. 

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AIM: To investigate the expression of estrogen receptors in myocardium of ovariectomized fe-male rats.METHODS: One week after bilateral ovariectomy, the female SD rats were divided into 3 groups randomly :OVX +estrogen (0.15 mg/kg,s.c.) , OVX +normal sodium and control .After 4 weeks of treatment, the expression of estrogen receptors in the myocardium were evaluated by Western blotting.RESULTS: After ovariectomy, serum estrogen de-creased significantly, compared with control[( 88±22 vs 403±59) pmol/L, P <0 .05], the expression of estrogen receptors decreased( P<0.05) .After estrogen replacement therapy, serum estrogen increased [( 3864±105) pmol/L], estrogen upregulated the ex-pression of ERβreceptor( P<0.05) and downregulat-ed ERαreceptor (P<0.05) in myocardium .CONCLUSION: Estrogen replacement modifies the expres-sion of estrogen receptors in myocardium of ovariecto-mized female rats.

Effects of Gypenosides on contraction of isolated duodenum smooth muscle of rabbits

ZHANG Wei-ping, LI Zheng-hong

2008, 13(4):  435-444. 

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AIM: To investigate the efftct of Gypenosides on contraction of isolated duodenum smooth mustle of rabbits. METHODS: The routine experimental method of isolated duodenal smooth muscle of rabbits were used. The contraction curves of isolated duodenum smooth mustle before and after administration of different concentrations of Gypenosides (0.01%-1.00%) were recorded by Medlab biological information collecting system. RESULTS: Different concentrations of Gypenosides significantly reduced thecontraction amplitude of isolated duodenum smooth mustle of rabbits, and showed a dose -dependent relationship. CONCLUSION: Gypenosides can inhibit the contraction of isolated duodenum smooth muscle of rabbits.

Efficacy and safety of gabapentin in the treatment of migraine: a double-blind randomized placebo-controlled study

GE Lin-tong, LIN Li, WU Hui-juan, ZHANG Jing

2008, 13(4):  445-448. 

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AIM: To evahuate the efficacy and safety of gabapentin in the treatment of migraine.METHODS: A randomized, double-blind, placebo-controlled study was taken.104 patients( aged 18 to 60 year) with migraine were randomly assigned to receive placebo( control group, 49 patients) or gabapentin( experimental group, 55 patients) for 4 weeks. The efficacy, side effects, TCD and EEG were assessed at the beginning of trials, and 4th， 8th weeks. RESULTS: Comparing with the placebo goup, the frequency，duration and intensity of migraine were markedly decreased in gabapentin group, there was significant difference between groups( P<0.01). The side effects did not differ between groups( P>0.05). CONCLUSION: Gabapentin is an effective and safe agent from migraine.

Effect of glargine combined aspart insulin when stroke happened in type 2 diabetes

YU Ping, LI Qiang, YANG Chun-xiao, SUN Yu-qian, LIANG Qing-cheng, ZHANG Jin-chao

2008, 13(4):  449-451. 

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AIM: To explored the combination of glargine and aspart in stoke of diabetes. METHODS: 40 patients were randomly assigned to two groups for insulin therapy, glargine combined aspart group (Agroup); three regular and one NPH( B group) group. Blood glucose was detected 7 times each day. RESULTS: Fasting and post meal blood glucose were more better in A group than B group after 7 days, and less hypoglycemia happened. ( Fasting 7.2±0.8, 9.4±1.9, PK 0.05; post meal, 9.0±1.8, 10.3±2.3,P<0.05, mmol/L). CONCLUSION: Glargine combined aspart w as more effective and has less hypoglycemia in diabetes when stroke happened.

Relationship between type 2 diabetes and retinol binding protein 4 and its genetic poly morphism

ZHOU Fang, ZHOU Hong-hao, LIU Zhao-qian

2008, 13(4):  452-456. 

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Retinol-binding potein ( RBP) is the primary transport protein for retionl. Serum plasma levels of RBP can serve as an index for detecting the development and the curative effects of the diseases of kindey and liver, as well as nutnitional diseases. Recent research reported that retinol binding protein 4(RBP4) is a newly discovered messenger fiom adipocyte, which plays a role in insulin resistance and obesity. The research and findings of the structure, gene polymorphism of RBP4, and association of RBP4 with type 2 diabetes ( T2DM) was reviewed in this article.

Advance in study of cardiovascular ion channel diseases

ZHENG Jian-fa, KE Yong-sheng

2008, 13(4):  457-462. 

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The cardiovascular ion channel disease is one of the main among ion channel diseases. It plays the important effect of cardiovascu lar disease. Ion channel diseases are responsible for almost all arrhythmias and sudden cardiac death. This text reviews genetic and acquired cardiac ion channel diseases, and associated trea tments are introduced briefly.

Effects of brain derived neurotrophic factor on ion channel

LI Guan-hua, FENG Ze-guo, ZHOU Jian-ping , WANG Hong

2008, 13(4):  463-467. 

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Brain derived neurotrophic factor ( BD-NF) is one of the family of neurotrophic factors (NTF) which plays an important role in the growth, develop-ment, differentiation, maintenance and regeneration of various types of neurons in the CNS and PNS .BDNF also can affect the resting membrane potential of neu-rons, nervous excitation and synaptic transmission .It plays a critical role in the induction of synaptic plastic-ity .Many of these effects of BDNF are mediated by its modulation of ion channel properties .Recent report have shown that BDNF not only acts as a modulation of ion channels following TrkB-mediated activation of in-tracellular second messenger cascades and protein phosphorylation, but can also directly opens a Na⁺ channel. Thus, in addition its role as a potent neuromodulator，BDNF emerges as an excitatory transmitter.

Discussions in pharmacological action and the development in clinical application of puer arin

YAO Dan, DING Xuan-sheng

2008, 13(4):  468-474. 

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As a saft and effective traditional Chinese medicine’s parenteral in jection, puerarin, whose molecular formula is 4,7-dihydroxy- 8β-D glucose isoflavone, has comprehensive and extensive phammaco logical action, and is generally used for treatment of cardiovascu lar and cerebrov ascular system diseases such as arhythmia, myocardial ischemia and hypertension.It has an extensive deve lopment perspective. The recent research in the mechanism of phamacological action and clinical application W ere review ed.