新生鼠脑缺氧缺血损伤时血红素加氧酶与细胞红蛋白表达的关系研究

中国儿童保健杂志 ›› 2012, Vol. 20 ›› Issue (9): 810-813.

新生鼠脑缺氧缺血损伤时血红素加氧酶与细胞红蛋白表达的关系研究

肖丹萍,史雪川,杨汉华

汕头大学医学院第二附属医院儿科,广东汕头 515000

收稿日期:2012-01-04 发布日期:2012-09-06 出版日期:2012-09-06
作者简介:肖丹萍(1984-),女,广东人,住院医师,硕士学位,主要研究方向为新生儿疾病
基金资助:
广东省科技计划项目(2008B680701036);广东省科技计划项目(B2009180)

Relation ship between heme oxygenase and cytoglobin expression on the hypoxia-ischemic brain damage in neonatal rat

XIAO Dan-ping,SHI Xue-chuan,YANG Han-hua

Department of Pediatrics,the Second Affiliated Hospital of Medical College,Shantou University,Shantou,Guangdong 515000,China

Received:2012-01-04 Online:2012-09-06 Published:2012-09-06

摘要/Abstract

摘要： 【目的】探讨血红素加氧酶(heme oxygenase,HO)与细胞红蛋白(cytoglobin,CYGB)表达的关系,为缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的防治提供实验依据。【方法】将7日龄新生SD大鼠170只随机分为正常对照组、假手术组、HIBD组、HIBD+Hemin组、HIBD+ZnPP组5组。其中HIBD组、HIBD+Hemin组及HIBD+ZnPP组在HIBD模型制备前12 h,分别腹腔注射生理盐水、Hemin及ZnPP,然后制备HIBD模型。术后于0、24、48 h采用免疫组化染色法分析HO -1及CYGB蛋白表达特点。【结果】缺氧后HO -1及CYGB表达随时间增长而递增,而且对于同一时间点HIBD+Hemin组HO -1及CYGB的表达均明显高于HIBD+ZnPP组,HIBD组居中,各组间比较差异有统计学意义;5组海马HO -1及CYGB表达均高度相关,0 h的r为0.687~0.916、24 h的r为0.473~0.903、48 h的r为0.659~0.962,皮质两者的表达亦有相同结果。【结论】 Hemin诱导HO-1能使CYGB高表达,而ZnPP抑制HO-1则使CYGB表达减少;HO -1及CYGB的表达在HIBD时存在极明显的正相关性;实验证明脑缺氧缺血损伤时HO -1对CYGB的表达可能具有一定的正性调控作用。

关键词: 细胞红蛋白, 血红素加氧酶, 缺氧缺血性脑损伤, 表达关系

Abstract: 【Objective】 To discuss the relation between the protein expression of heme oxygenase-1(HO-1) and cytoglobin (CYGB),and whether hemin would reduce the brain damage against hypoxic-ischemic damage. 【Methods】 SD rats of 7-day old were divided into 5 groups randomly,including control group,sham-operated group,HIBD group,HIBD +Hemin group,and HIBD+ZnPP group.The rats of HIBD+Hemin group,HIBD group and HIBD+ZnPP group were given Hemin,normal saline or ZnPP 12 hours before the operation.The rats of three last groups were made for HIBD model.All the groups were killed at 0 h,24 h,48 h after HIBD.At 0 h,24 h,48 h after hypoxic-ischemic,observed the protein expression of HO-1 and CYGB in the brain. 【Results】 The protein expression of HO-1 and CYGB in all HIBD model groups were increased as time gone on.At any time after hypoxic-ischemic brain damage the expression of HO-1 and CYGB in the HIBD model groups were more than those in sham-operated group and control group.And in three HIBD model groups the expression of HO-1 and CYGB were showed by immunohistochemistry:HIBD+Hemin group>HIBD group>HIBD+ZnPP group,comparison of the three groups was with significant difference.The expression of HO-1 and CYGB had positive correlations in contex and hipppcampi in five groups.The relative factors r at 0h after HIBD were 0.687~0.916,24 h after HIBD were 0.473~0.903,48 h after were 0.659~0.962.The contex also had similar result. 【Conclusions】 Hemin which was the abduction of HO-1 could increase the expression of HO-1 in contex and hippocampus of HIBD+Hemin group.ZnPP which was the depressor of HO could decrease the expression of HO-1 of the HIBD+ZnPP group.The expression of HO-1 and CYGB was consistency,and they may have clear positive correlation.

Key words: cytoglobin, heme oxygenase-1, hypoxic-ischemic brain damage, relation

中图分类号:

Q95-33

肖丹萍,史雪川,杨汉华. 新生鼠脑缺氧缺血损伤时血红素加氧酶与细胞红蛋白表达的关系研究[J]. 中国儿童保健杂志, 2012, 20(9): 810-813.

XIAO Dan-ping,SHI Xue-chuan,YANG Han-hua. Relation ship between heme oxygenase and cytoglobin expression on the hypoxia-ischemic brain damage in neonatal rat[J]. journal1, 2012, 20(9): 810-813.

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