Abstract: Objective To investigate the etiology of infectious diarrhea in Yinchuan children and provide the basis for clinical rational drug use. Methods During April 2012 to April 2013 the Ningxia People's Hospital outpatient and hospitalized children with diarrhea patients initial diagnosis had the fresh fecal bacterial culture,and the resulting samples were tested by RT-PCR technology for rotavirus (HRV),norovirus (NORV),bar-like viruses (SPAV),astrovirus (ASTV) and enteric adenovirus (EADV). Results A total of 286 stool samples were detected,208 cases found pathogen with detection rate of 72.73%.Virus detection,the positive rate of 172 cases was 60.14%,which HRV 84 cases (29.34%),NORV 62 cases (21.77%),SPAV 14 cases (4.90%),ASTV 27 cases (9.44%),EADV 21 cases (7.34%).The detection rate of bacterial culture pathogens was 12.58%(36 cases).Salmonella infection was more common.Mixed infection was 36 cases (12.58%). Conclusion Yinchuan infectious diarrhea in children is connected with viral infections,HRV,NORV as the main pathogens.

Key words: diarrhea, pathogen detection, reverse transcription-polymerase chain reaction

摘要： 目的 探讨银川地区儿童感染性腹泻病病原学分布特点,为临床合理用药提供依据。方法 采集2012年4月-2013年4月期间就诊宁夏人民医院门诊及住院的初步诊断腹泻病患儿的新鲜粪便进行细菌培养,同时对所得样本采用RT-PCR技术进行轮状病毒(HRV)、诺如病毒(NORV)、扎如病毒(SPAV)、星状病毒(ASTV)及肠道腺病毒(EADV)检测。结果 286份粪便样本共检测出致病菌208例,检出率72.73%。病毒检测172例阳性,阳性率 60.14%,其中HRV84例(29.34%)、NORV62例(21.77%),SPAV14例(4.90%)、ASTV27例(9.44%),EADV 21例(7.34%)。细菌培养致病菌36例,检出率为12.58%,以沙门氏菌感染多见。混合感染36例(12.58%)。结论 银川地区儿童感染性腹泻病以病毒感染为主,HRV、NORV为主要致病菌。

关键词: 腹泻, 致病菌检测, 逆转录-聚合酶链反应