Abstract: 【Objective】 To study the characters of some cytokines levels in newborn with sepsis and infection. 【Method】 Cytokines were measured by sanwich enzyme-linked immunosorbent assay and nitric acid deoxidize method. 【Results】 The plasma IL-6, IL-8 and TNF-α levels in newborn with sepsis were significantly higher than the health control group (P<0.01). The plasma IL-6 levels in common infection had no difference with the health control group(P>0.05). There were no difference of TNFα between infection with hypoxic-ischemic encephalopathy(HIE) and pure infection(P>0.05), the levels of IL-6 were lower(P<0.01) and NO were higher (P<0.01)in the firster. 【Conclusions】 The high levels of IL-6, TNF-α and NO in newborn plasma indicate infection. TNF-α might be sensitive markers of diagnosis and degree of neonatal infection. The levels of IL-6, NO care be affected by asphyxia of newborn.

Key words: IL-6, TNF-α, NO, infection, newborn

摘要： 【目的】 分析比较新生儿感染性疾病(败血症与一般感染) 血浆中部分细胞因子的变化特征。 【方法】 采用双抗体夹心酶联免疫吸附实验法(ELISA) 测定白细胞介素6 (interleukin, IL-6) 、肿瘤坏死因子α(tumer necrosis factor, TNF-α) 的水平,使用硝酸还原酶法测定NO水平。 【结果】 败血症患儿血浆IL-6、TNF-α、NO水平显著高于健康对照组(P<0.01),一般感染患儿血浆IL-6水平与健康对照组相比,差异无统计学意义( P>0.05)。新生儿缺血缺氧性脑病(hypoxic-ischemic encephalopathy,HIE)合并感染组TNF-α较单纯感染组差异无统计学意义(P>0.05),IL-6水平降低(P<0.01),而NO水平显著增高(P<0.01)。 【结论】 新生儿血浆中高水平的IL-6、TNF-α、NO提示机体存在感染, TNF-α可做为早期诊断的灵敏指标,并与感染轻重程度有关。新生儿窒息缺氧可影响IL-6和NO的水平。

关键词: 白细胞介素6, 肿瘤坏死因子α, NO, 感染, 新生儿