Relationship between early catch-up growth and insulin sensitivity of intrauterine growth retardation rats

doi:10.11852/zgetbjzz2015-23-06-11

Abstract

Abstract: Objective To investigate the relationship between catch-up growth at early stage and insulin sensitivity of intrauterine growth retardation (IUGR) rat. Method An intrauterine growth retardation (IUGR) animal model was established by protein malnutrition during the mother's late pregnancy.Appropriate growth was defined by a weight within one SD of the colony mean,named as IUGR catch up growth group(IUGR CG),weight which exceed the above standerd named as IUGR over catch up group (IUGR OCG).Weight growth rate was calculated by standerd deviation score (SDS).At 21d,2 months and 4 months after birth,offspring rats were fasted for 12 h and then angular vein blood was collected to measure fasting plasma glucose (FPG) and fasting serum insulin level,then insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI) were calculated.IUGR hyperglycemia group (IUGR HG) was defined by 95% limit controls of FPG. Results 1)The mean offspring birth weight of IUGR group was (4.37±0.69)g,which was lower than that of control group (7.03±0.55)g(t=-20.75,P<0.05).In IUGR group,the incidence of IUGR offspring was 93.33%.2)HOMA-IR was significantIy higher in IUGR rats than that in the control group at the age of 2 months to 4 months (P<0.05).ISI was significantly lower at 2 months of age and continued to 4m (P<0.05).3) FPG of IUGR OG was significantly higher than that of IUGR CG and control group at 2 months (P<0.05).4) The growth rate of IUGR rat at 7d was the fastest,which was statistically highly correlated with insulin resistance index of 2 month and 4 month (P<0.05). Conclusion The more faster catch up growth at early age,the IUGR rats are more prone to have insulin resistance at adulthood.

Key words: intrauterine growth retardation, catch up growth, insulin resistance, rat

摘要： 目的探讨宫内发育迟缓大鼠早期追赶生长与生后胰岛素敏感性的关系。方法孕后期低蛋白限制饮食法建立宫内发育迟缓模型。分别于仔鼠生后21天、2月龄、4月龄,采用美国强生稳步型血糖仪快速测定血浆葡萄糖。采用酶联免疫吸附试验测定血清胰岛素,计算胰岛素敏感指数和胰岛素抵抗指数。结果 1)低蛋白组新生仔鼠平均出生体重为(4.37±0.69)g,低于对照组的(7.03±0.55)g,差异有统计学意义(t=-20.75,P<0.05)。低蛋白组仔鼠中宫内发育迟缓(fetal growth retardation,IUGR)的发生率为93.33%(70/75)。2)生后2月龄始,低蛋白组空腹血糖、空腹胰岛素、胰岛素抵抗指数均较对照组有所增加,胰岛素敏感性相对降低,直至4月龄,差异有统计学意义(P<0.05)。3)生后2月龄,IUGR 追赶生长组空腹血糖最高,三组间空腹血糖差异均有统计学意义(P<0.05)。4)IUGR组生后7天生长速度最快,且与2月龄、4月龄胰岛素抵抗指数呈高度正相关,均具有统计学意义(P<0.05)。结论宫内发育迟缓大鼠早期追赶生长越快,成年期越容易发生胰岛素抵抗。

关键词: :宫内发育迟缓, 追赶生长, 胰岛素抵抗, 大鼠

CLC Number:

Q95-33

ZHANG Jin,ZHANG Hui,XING Yan,WANG Xin-li. Relationship between early catch-up growth and insulin sensitivity of intrauterine growth retardation rats[J]. journal1, 2015, 23(6): 595-598.

张金,张慧,邢燕,王新利. 宫内发育迟缓大鼠早期追赶生长与胰岛素敏感性变化的关系[J]. 中国儿童保健杂志, 2015, 23(6): 595-598.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/10.11852/zgetbjzz2015-23-06-11

https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/Y2015/V23/I6/595

References

[1] Szostak-Wegierek D,Szamotulska K.Fetal development and risk of cardiovascular diseases and diabetes type 2 in adult life[J].Med Wieku Rozwoj,2011,15(3 Pt 1):203-215.
[2] Saenger P,Czernichow P,Hughes I,et al.Small for gestational age:short stature and beyond[J].Endocrine Reviews,2007,28(2):219-251.
[3] Karlberg J,Albertsson-Wikland K.Growth in full-term small-for-gestational-age infants:from birth to final height[J].Pediatr Res,1995,38(5):733-739.
[4] Eriksson JG,Osmond C,Barker DJ et al.Patterns of growth among children who later develop type 2 diabetes or its risk factors[J].Diabetologia,2006,49(12):2853-2858.
[5] Soto N,Bazaes RA,Pea V,et al.Insulin sensitivity and secretion are related to catch-up growth in small-for-gestational-age infants at age 1 year:results from a prospective cohort[J].J Clin Endocrinol Metab,2003,88(8):3645-3650.
[6] Martin-Gronert MS,Tarry-Adkins JL,Cripps RL,et al.Maternal protein restriction leads to early life alterations in the expression of key molecules involved in the aging process in rat offspring[J].Am J Physiol Integr Cornp Physiol,2008,294(2):494-500.
[7] 关育红,王新利,崔蕴璞,等.宫内发育迟缓大鼠胰岛素敏感性的动态变化[J].中华围产医学杂志,2011,14(4):221-226.
[8] Thamotharan M,Shin BC,Suddirikku DT,et al.GLUT4 expression and subcellular localization in the intrauterine growth-restricted adult rat female offspring[J].Am J Physiol Endocrinol Metab,2005,288(5):935-947.
[9] Günz H,Bundak R,Furman A,et al.Z-score reference values for height in Turkish children aged 6 to 18 years[J].J Clin Res Pediatr Endocrinol,2014,6(1):28-33.
[10] Hermann GM,Miller RL,Erkonen GE,et al.Neonatal catch up growth increases diabetes susceptibility but improves behavioral and cardiovascular outcomes of low birth weight male mice[J].Pediatr Res,2009,66(1):53-58.
[11] World Health Organization.Laboratory diagnosis and monitoring of diabetes mellitus[R].Hans Reinauer Philip D Home,2002,11-14.
[12] Chakravarthy MV,Zhu Y,Wice MB,et al.Decreased fetal size is associated with-cell hyperfunction in early life and failure with age[J].Diabetes,2008,57(10):2698-2707.
[13] LI Yao,XIN Ying.Expression of insulin receptor substrates of pancreas,liver and skeletal muscle in intauterine growth retardation rats[J].Chin J Evid Based Pediatr,2011,5(6):225-229.
[14] Fagerberg B,Bondjers L,Nilsson P.Low birth weight in combination with catch-up growth predicts the occurrence of the metabolic syndrome in men at late middle age:the Atherosclerosis and Insulin Resistance study[J].J Intern Med,2004,256(3):254-259.
[15] Hales CN,Barker DJ,Clark PM,et al.Fetal and infant growth and impaired glucose tolerance at age 64[J].BMJ,1991,303(6):1019-1022.
[16] Hovi P,Andersson S,Eriksson JG,et al.Glucose regulation in young adults with very low birth weight[J].N Engl J Med,2007,356(20):2053-2063.
[17] Veening MA,Van Weissenbruch MM,Delemarre-Van De Waal HA.Glucose tolerance,insulin sensitivity,and insulin secretion in children born small for gestational age[J].J Clin Endocrinol Metab,2002,87(10):4657-4661.
[18] Baik M,Rajasekar P,Lee MS,et al.An intrauterine catch-up growth regimen increases food intake and post-natal growth in rats[J].J Anim Physiol Anim Nutr (Berl),2014,98(6):1132-1141.
[19] 陈璐璐,汪晓芬,郑娟,等.大鼠追赶生长早期脂肪组织和骨骼肌葡萄糖代谢差异及其机制 [J].中华内分泌代谢杂志,2008,24(3):244-247.
[20] Brown L.Endocrine regulation of fetal skeletal muscle growth:impact on future metabolic health[J].J Endocrinol,2014,19(2):209-220.
[21] Rotteveel J,van Weissenbruch MM,Delemarre-Van de Waal HA.Decreased insulin sensitivity in small for gestational age males treated with GH and preterm untreated males:a study in young adults[J].Eur J Endocrinol,2008,158(6):899-904.