Abstract: Objectives To explore the effects and clinical significance of prenatal taurine on IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of intrauterine growth restriction(IUGR). Methods IUGR was induced by food restriction throughout pregnancy.Pregnant rats were randomly divided into 3 groups:control group,IUGR group and taurine group[300 mg/(kg·d)].The expressions of serum IL-6,IL-8,TNF-α and IL-1β in brains were detected by Enzyme linked immunosorbent- assay(ELISA). Results The expressions of IL-6 in control group,test group and taurine group were:(81.070±5.286)pmol/L,(148.362±18.733)pmol/L and (107.361±11.184)pmol/L,there were significant differences among the three groups (H=43.760,P=0.00).The contents of IL-8 in three groups were:(90.573±7.344)pmol/L,(165.596±8.635)pmol/L and (126.309±11.008)pmol/L.There were significant differences among the three groups (H=45.910,P=0.00).The expressions of TNF-α in three groups were:(8.231±0.607)pmol/L,(14.881±0.703)pmol/L and (11.709±0.936)pmol/L,there were significant differences among the three groups (H=45.929,P=0.00).The expressions of IL-1β in three groups were:(46.693±4.122)pmol/L,(84.799±4.315)pmol/L and (62.407±4.631)pmol/L,there were significant differences among the three groups (H=46.064,P=0.00). Conclusion Prenatal taurine can reduce expressions IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of IUGR.

Key words: intrauterine growth restriction, fetal rat, taurine, interleukin-6, interleukin-8, tumor necrosis factor alpha, interleukin-1β

摘要： 目的 了解宫内生长受限(IUGR)胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β表达水平,探讨孕鼠补充牛磺酸对其表达水平的影响及临床意义。方法 采用妊娠期全程饥饿法建立IUGR胎鼠模型,将15只孕鼠随机分为3组正常对照组、IUGR组、IUGR＋补充牛磺酸组[300 mg/(kg·d)](牛磺酸组)。采用酶联免疫吸附法测定三组胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β的含量。结果 对照组、IUGR组、牛磺酸组胎鼠脑组织IL-6含量分别为(81.070±5.286)pmol/L、(148.362±18.733)pmol/L和(107.361±11.184)pmol/L,差异有统计学意义(H=43.760,P=0.00);三组胎鼠脑组织IL-8含量分别为(90.573±7.344)pmol/L、(165.596±8.635)pmol/L和(126.309±11.008)pmol/L,差异有统计学意义(H=45.910,P=0.00);三组胎鼠脑组织TNF-α含量分别为(8.231±0.607)pmol/L、(14.881±0.703)pmol/L和(11.709±0.936)pmol/L,差异有统计学意义(H=45.929,P=0.00);三组胎鼠脑组织IL-1β含量分别为(46.693±4.122)pmol/L、(84.799±4.315)pmol/L和(62.407±4.631)pmol/L,差异有统计学意义(H=46.064,P=0.00)。结论 孕鼠补充牛磺酸可减少IUGR胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β的表达。

关键词: 宫内生长受限, 胎鼠, 牛磺酸, 白介素-6, 白介素-8, 肿瘤坏死因子-α, 白介素-1β