Abstract: Objective To analyze the effect of different types of allergens on T lymphocyte subsets of children with asthma.Methods Totally 147 cases of asthma children were collected to detect the allergens and flow cytometry was conducted to test immune indices levels, such as CD3, CD4, CD8, CD4/CD8.Results Children developed asthma at the age of one year and eleven months averagely. The proportion of male cases (72.1%) was higher than that of female cases (27.9%). Environmental allergy was found predominant in childhood with allergic asthma. The CD4 level of children with environmental allergy was significantly different from that without environmental allergy (χ²=10.317, P=0.006), while significant difference was not found in term of CD3, CD4, CD8, CD4/CD8 levels (P>0.05).Conclusion Environmental allergy is prone to influence children′s immune function, resulting in CD4 T lymphocyte out of balance. So it is supposed to pay more attention to environmental allergy in judging the immune function of asthma children and clinical prevention and treatment.

Key words: asthma, allergen, T Lymphocyte subsets, children

摘要： 目的 分析不同类型过敏原对哮喘患儿T淋巴细胞亚群的影响,以期为哮喘防治提供参考。方法 对147例支气管哮喘急性发作患儿进行过敏原筛查,应用流式细胞术检测其CD3、CD4、CD8、CD4/CD8水平。结果 本研究中哮喘患儿平均发病年龄为1岁11个月,男性患儿(72.1%)较女性患儿(27.9%)多,儿童过敏性哮喘以环境过敏阳性为主。环境过敏原阳性组外周血CD4水平与过敏原阴性患儿之间的差异有统计学意义(χ²=10.317,P=0.006)。不同类型过敏原哮喘患儿外周血CD3、CD8、CD4/CD8之间的差异无统计学意义(P>0.05)。结论 环境过敏原致敏更易影响哮喘儿童免疫功能的改变,造成CD4 T淋巴细胞平衡失调,在哮喘患儿免疫功能判断和临床防治中应重点关注。

关键词: 哮喘, 过敏原, T淋巴细胞亚群, 儿童