Bioinformatics analysis of the regulation of miR-429 towards target genes in tic disorders

doi:10.11852/zgetbjzz2022-0746

Abstract

Abstract: Objective To predict tic disorders (TD) related target genes of microRNA (miRNA, miR)-429 by using bioinformatics analysis, and to take intersection between the target genes of miR-429 and TD related genes (TRGs), so as to further understand the impaction of miR-429 in TD. Method MiRbase was used to obtain the sequences of mature miR-429 in multiple species for conserved analysis.GeneCards database and DisGeNET database were used to acquire TRGs.MiRDB database and DIANA-microT were used to gain the target genes of miR-429.TD related target genes were obtained by intersection of the target genes gained by MiRDB database and DIANA-microT with TRGs.RNAhybird database was used to predict the sequences and minimum free energy (MFE) of hybridization.Functional enrichment analysis of TD related target genes was performed by DAVID database. Results Only 1 nucleotides among the 22 nucleotides of miR-429 was different among specie.A total of 9 genes were identified as TD related target genes.The sequences of hybridization of ASH1L and SLITRK1 with miR-429 did not include the nucleotide that differed among species, indicating that the two hybird pairs were more conserved.The first gene with the smallest MFE of hybridization were CHD2 (-25.0 kcal/mol).The first terms in each category of functional enrichment analysis were adult behavior of biological process, glutamatergic synapse of cell components and chromatin binding of molecular functions.No statistically significant pathway enrichment was obtained. Conclusion MiR-429 might impact behaviors in TD by regulating glutamatergic synapses via related genes, especially SLITRK1.

Key words: miR-429, glutamatergic, SLITRK1 gene, bioinformatics analysis, tic disorders

摘要： 目的通过生物信息学分析,并将miR-429靶基因与抽动障碍(TD)相关基因(TRGs)关联,预测TD相关靶基因,以更好地理解miR-429在TD中的作用。方法通过miRbase数据库获取多物种成熟miR-429的序列,进行保守性分析。通过GeneCards和DisGeNET数据库获取TRGs,并将miRDB数据库及DIANA数据库的microT工具预测的miR-429的靶基因与TRGs取交集获得TD相关靶基因。运用RNAhybird数据库对杂交的位点序列和最小自由能(MFE)进行预测。通过DAVID数据库对TD相关靶基因进行功能富集分析。结果 miR-429的22个核苷酸中仅2个核苷酸在物种间存在差异,总共获得9个基因为TD相关靶基因。仅ASH1L、SLITRK 1 与miR-429杂交的序列中未包含物种间存在差异的核苷酸,最小的杂交MFE的基因为CHD2(-25.0 kcal/mol)。功能富集分析各分类的首个功能术语为生物学过程的成人行为,细胞组分的谷氨酸能突触,以及分子功能的染色质结合。未获得有统计学意义的通路富集。结论 miR-429可能通过相关基因调控谷氨酸能突触而影响TD行为,尤其是SLITRK1基因。

关键词: miR-429, 谷氨酸能, SLITRK1基因, 生物信息学分析, 抽动障碍

CLC Number:

R749.94

JIANG Jilong, CHEN Yanhui, HUANG Huifang, HUANG Yuxian. Bioinformatics analysis of the regulation of miR-429 towards target genes in tic disorders[J]. Chinese Journal of Child Health Care, 2023, 31(3): 268-273.

江极龙, 陈燕惠, 黄惠芳, 黄玉娴. miR-429对抽动障碍靶基因调控的生物信息学分析[J]. 中国儿童保健杂志, 2023, 31(3): 268-273.

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