lncRNA PCAT19吸附miR-142-5p调控ING3基因表达抑制鼻咽癌细胞的增殖和侵袭

doi:10.12092/j.issn.1009-2501.2020.07.001

中国临床药理学与治疗学 ›› 2020, Vol. 25 ›› Issue (7): 721-727.doi: 10.12092/j.issn.1009-2501.2020.07.001

• 基础研究 • 下一篇

lncRNA PCAT19吸附miR-142-5p调控ING3基因表达抑制鼻咽癌细胞的增殖和侵袭

侯彬¹，黄维平¹，尹中普¹，胡守森²

¹南阳市中心医院耳鼻喉科，南阳 473000，河南；²郑州大学第一附属医院耳鼻喉科，郑州 450052，河南

收稿日期:2020-02-10 修回日期:2020-06-17 出版日期:2020-07-26 发布日期:2020-07-31
通讯作者: 黄维平，通信作者，男，硕士，主任医师，研究方向：鼻咽癌恶性转化的分子机制。 E-mail: qiye56@126.com
作者简介:侯彬，男，硕士，主治医师，研究方向：lncRNA对鼻咽癌生长和转移的影响。 E-mail: 87561678@qq.com
基金资助:
国家自然科学基金（81600812）

lncRNA PCAT19 inhibits the proliferation and invasion of nasopharyngeal carcinoma cells by adsorbing miR-142-5p and regulating the expression of ING3 gene

HOU Bin ¹, HUANG Weiping¹, YIN Zhongpu¹, HU Shousen ²

¹Department of Otolaryngology, Nanyang Central Hospital, Nanyang 473000, Henan, China; ²Department of Otolaryngology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China

Received:2020-02-10 Revised:2020-06-17 Online:2020-07-26 Published:2020-07-31

摘要/Abstract

摘要： 目的：检测长链非编码RNA（long non-coding RNA, lncRNA）PCAT19在鼻咽癌组织和细胞株中的表达量，探讨其抑制鼻咽癌细胞增殖和侵袭的分子作用机制。方法：实时荧光定量PCR（qRT-PCR）法检测鼻咽癌组织和5种鼻咽癌细胞株中PCAT19的相对表达。在表达最低的鼻咽癌细胞株转染空载质粒（对照组）或高表达PCAT19质粒（实验组）。qRT-PCR检测转染效率。MTS法和Transwell侵袭实验分别检测高表达PCAT19对鼻咽癌细胞增殖能力和侵袭能力的影响。生物信息学预测PCAT19的靶基因。qRT-PCR和Western blot检测靶基因在mRNA和蛋白水平的表达。结果：与慢性鼻咽炎组织比较，PCAT19在鼻咽癌组织的表达降低（P<0.01）。与永生化鼻咽上皮细胞比较，5种鼻咽癌细胞株PCAT19的表达均明显降低（P<0.05），SUNE-1细胞中的表达最低（P<0.01）。转染高表达PCAT19质粒可明显促进PCAT19的表达（P<0.01）。高表达PCAT19可抑制鼻咽癌SUNE-1细胞的增殖能力（P<0.01）和侵袭能力（P<0.01）。PCAT19的靶基因是miR-142-5p，miR-142-5p的靶基因生长抑制因子3（inhibitor of growth gene 3, ING3）。高表达PCAT19后，miR-142-5p表达明显降低（P<0.01），而ING3在mRNA和蛋白水平上的表达均明显增加（P<0.01）。结论：鼻咽癌组织和细胞株中PCAT19的表达下调。上调PCAT19可抑制鼻咽癌SUNE-1细胞的增殖和侵袭能力，其作用机制可能是PCAT19通过吸附miR-142-5p促进ING3基因的表达。

关键词: 长链非编码RNA, 鼻咽癌, 生长抑制因子3, 细胞增殖, 细胞侵袭

Abstract: AIM: To investigate the expression of long non-coding RNA (lncRNA) PCAT19 in nasopharyngeal carcinoma tissues and cell lines, and to explore its molecular mechanism of inhibiting proliferation and invasion of nasopharyngeal carcinoma cells. METHODS: Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the relative expression of PCAT19 in nasopharyngeal carcinoma tissues and five nasopharyngeal carcinoma cell lines. The lowest expressing nasopharyngeal carcinoma cell line was transfected with empty plasmid (control group) or high expression PCAT19 plasmid (experimental group). qRT-PCR was used to detect transfection efficiency. MTS method and Transwell invasion test were used to detect the effect of overexpressing PCAT19 on the proliferation and invasion ability of nasopharyngeal carcinoma cells. Bioinformatics predicts target genes for PCAT19. qRT-PCR and Western blot were used to detect target gene expression at mRNA and protein levels. RESULTS: Compared with adjacent tissues, the expression of PCAT19 in nasopharyngeal carcinoma tissues was decreased (P<0.01). Compared with immortalized nasopharyngeal epithelial cells, the expression of PCAT19 in five nasopharyngeal carcinoma cell lines was significantly reduced (P<0.05), and the expression was the lowest in SUNE-1 cells (P<0.01). Transfection of high-expressing PCAT19 plasmid could significantly promote the expression of PCAT19 (P<0.01). High expression of PCAT19 could inhibit the proliferation ability (P<0.01) and invasive ability (P<0.01) of nasopharyngeal carcinoma SUNE-1 cells. The target gene of PCAT19 was miR-142-5p, the target gene of miR-142-5p was inhibitor of growth gene 3 (ING3). After high expression of PCAT19, miR-142-5p expression was significantly reduced (P<0.01), and the expression of ING3 at both mRNA and protein levels was significantly increased (P<0.01). CONCLUSION: PCAT19 expression is down-regulated in nasopharyngeal carcinoma tissues and cell lines. Up-regulating PCAT19 can inhibit the proliferation and invasion of nasopharyngeal carcinoma SUNE-1 cells. The mechanism may be that PCAT19 promotes the expression of ING3 gene by adsorbing miR-142-5p.

Key words: long non-coding RNA, nasopharyngeal carcinoma, inhibitor of growth gene 3, cell proliferation, cell invasion

中图分类号:

R739.63

侯彬, 黄维平, 尹中普, 胡守森. lncRNA PCAT19吸附miR-142-5p调控ING3基因表达抑制鼻咽癌细胞的增殖和侵袭[J]. 中国临床药理学与治疗学, 2020, 25(7): 721-727.

HOU Bin, HUANG Weiping, YIN Zhongpu, HU Shousen. lncRNA PCAT19 inhibits the proliferation and invasion of nasopharyngeal carcinoma cells by adsorbing miR-142-5p and regulating the expression of ING3 gene[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(7): 721-727.

[1]	金乐, 蒋多晨, 陈洪晓, 刘苏, 陈昭琳, 居靖. 槲皮素通过GAS5调控GSK-3β/β-catenin逆转乳腺癌阿霉素耐药[J]. 中国临床药理学与治疗学, 2021, 26(12): 1344-1351.
[2]	宋丹丹, 陈雪源, 陈瑞琪, 李涵乔, 吴甜. 长链非编码RNA MALAT1在乳腺癌中的研究现状[J]. 中国临床药理学与治疗学, 2021, 26(1): 49-57.
[3]	汪向海, 邢敏, 吕镗烽, 刘红兵, 宋勇. 敲降长链非编码RNA 00467表达对肺腺癌患者预后的影响及其机制[J]. 中国临床药理学与治疗学, 2020, 25(8): 841-849.
[4]	王子元, 孙明瑜. 长链非编码RNA在结直肠癌耐药中的研究进展[J]. 中国临床药理学与治疗学, 2020, 25(7): 796-802.
[5]	刘建辉, 周根鸿, 李春林, 鲍美华, 刘方怡. 生物信息学筛选骨髓增生异常综合征相关长链非编码RNA[J]. 中国临床药理学与治疗学, 2020, 25(12): 1351-1356.
[6]	沈彬彬，周俊，黎亮，陆宁，姚明. 长链非编码RNA TSIX通过靶向miR-384调控人胰腺癌细胞增殖的研究[J]. 中国临床药理学与治疗学, 2018, 23(6): 621-626.
[7]	高元峰，李珊，刘林，欧阳荣. 胶质瘤中异常表达的lncRNAs的研究进展[J]. 中国临床药理学与治疗学, 2018, 23(5): 586-591.
[8]	胡洋，李智星，王果，朱院山. 长链非编码RNA与结直肠癌耐药的研究进展[J]. 中国临床药理学与治疗学, 2018, 23(4): 456-463.
[9]	陈薪薪，陈朝生. 长链非编码RNA与系统性红斑狼疮的研究进展[J]. 中国临床药理学与治疗学, 2018, 23(4): 471-476.
[10]	李智星，胡洋，王果，朱院山. lncRNA与Wnt/β-catenin通路的相互关系在结直肠癌中的研究进展[J]. 中国临床药理学与治疗学, 2018, 23(3): 342-351.
[11]	蔡民，许浏，沈兰，张杰. 长链非编码RNA FOXN3-AS2在肝癌中的表达及其对肝癌细胞增殖和侵袭的影响[J]. 中国临床药理学与治疗学, 2018, 23(11): 1246-1251.
[12]	孙跃胜，屈强，潘江华，童晓春，陈恩德，屈健. 长链非编码RNA在结直肠癌中的作用[J]. 中国临床药理学与治疗学, 2017, 22(2): 222-227.
[13]	张蕊，邓俊丽，孙红，王果，朱院山. 长链非编码RNA对肿瘤转移调控的研究进展[J]. 中国临床药理学与治疗学, 2016, 21(9): 1061-1067.

lncRNA PCAT19吸附miR-142-5p调控ING3基因表达抑制鼻咽癌细胞的增殖和侵袭

lncRNA PCAT19 inhibits the proliferation and invasion of nasopharyngeal carcinoma cells by adsorbing miR-142-5p and regulating the expression of ING3 gene

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价