Inhibitory action of crocetin on the expression of E-selectin in bovine endothelial cells induced by advanced glycation end products

Abstract

Abstract: AIM: To study the inhibitory effect of crocetin on the expression of E-selectin in bovine endothelial cells (BECs) induced by advanced glycation end products (AGEs).METHODS: Neutrophils and monocytes were separated and purified from the whole blood of new-born calf. The adhesion of neutrophils/ monocytes to endothelial cells was assayed by Fuhong staining method. Cell-based ELISA and sABC immunocytochemistry were adopted to analyze the expression of E-selectin.RESULTS: AGEs(100 μg/mL)could increase the adhesion of neutrophils/monocytes to endothelial cells(P<0.01 vs control). However, after pre-incubation the BECs with crocetin(1,0.1,0.01 μmol/L) for 12 h before exposure to AGEs (100 mg/mL), the above adhesion rate decreased significantly in a dose-dependent manner( P<0.05 or 0.01 vs AGEs group). Cell-based ELISA results demonstrated that the expression of E-selectin elevated in BECs treated by AGEs within 4 h, then decreased and regained to the normal level at 8 h, while the protein expression of E-selectin was suppressed by crocetin. sABC immunocytochemistry also confirmed that crocetin could decrease the level of E-selectin.CONCLUSION: These results demonstrate that crocetin could inhibit E-selectin over-expression in BECs exposed to AGEs and then down-regulate the adhesion of neutrophils/monocytes to endothelial cells, which is one of the possible mechanisms for crocetin to attenuate diabetic vascular complications.

Key words: Crocetin, Adhesion, E-selectin , Neutrophil, Monocyte, Endothelial cell

CLC Number:

R965.2

XIANG Min, ZHOU Cheng-hua, QIAN Zhi-yu. Inhibitory action of crocetin on the expression of E-selectin in bovine endothelial cells induced by advanced glycation end products[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 764-769.

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