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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2018, Vol. 23 ›› Issue (6): 640-645.doi: 10.12092/j.issn.1009-2501.2018.06.007

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Inhibitory effect and mechanism of Adina rubella flavonoids on platelet aggregation and thrombosis

FANG Qingxia 1,2,ZOU Xiaozhou 1,2,YU Wenying 3,FANG Jie 3,MO Li 1,3,YU Chenhuan 3   

  1. 1 Department of Pharmacy, Zhejiang Provincial People's Hospital, Hangzhou 310053, Zhejiang, China; 2 People's Hospital of Hangzhou Medical College, Hangzhou 310014, Zhejiang,China; 3 Zhejiang Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang, China
  • Received:2017-09-27 Revised:2017-11-24 Online:2018-06-26 Published:2018-06-19

Abstract:

AIM: To investigate the effect and mechanism of Adina rubella flavonoids (ARF) on platelet aggregation and activation. METHODS: Different stimulus[adenosine 5'diphosphate (ADP),arachidonic acid (AA), platelet activating factor (PAF)]were used to induce platelet aggregation in vitro. A rat model of acute pulmonary embolism caused by ADP, a mouse model of bleeding time and a rat model of arteriovenous shunt thrombosis were established to observe the inhibitory effect of ARF on platelet aggregation and thrombosis. The expression of CD41 and the activation of PI3K/Akt signal pathway were illustrated by Western blot. RESULTS: Compared with the control group, all ARF groups of the low-dose, middle-dose and high-dose significantly improved the survival rates of acute pulmonary embolism rats, prolonged the bleeding time and inhibited arteriovenous shunt thrombosis formation. ARF also inhibited platelet aggregation, and up-regulated expression of CD41 and the activation of PI3K/Akt signal pathway induced by ADP. CONCLUSION: ARF significantly inhibits the platelet aggregation and thrombosis, the mechanism may be related to CD41/PI3K/Akt signaling pathway.

Key words: Adina rubella, flavonoids, platelet aggregation, thrombus, signaling pathway

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