关键词: 肺癌, 跨膜蛋白106A

Abstract:

AIM: To investigate the expression of transmembrane protein 106A (TMEM106A) in lung cancer and its relationship with clinicopathological features, and the effect of this molecule on the proliferation of lung cancer cells. METHODS: Thirty-one cases of lung cancer tissues were collected from patients with early stage lung cancer. Tissue RNA was extracted and real-time quantitative PCR was used to detect the relative expression of TMEM106A mRNA in lung cancer tissues and para-cancer tissues. TMEM106A lentiviral expression vector Plenti-UBI/TMEM106A was constructed and transfected into H1299 cells to overexpress TMEM106A. Cell proliferation assay and colony formation assay were used to observe the effect of TMEM106A overexpression on the proliferation of lung cancer H1299 cells.RESULTS:Real-time quantitative PCR (qRT- PCR) showed that the relative expression of TMEM106A gene mRNA in lung cancer tissues was lower than that in the control para-cancer tissues. H1299-TMEM106A，the cell line overexpressed TMEM106A, was successfully constructed. Compared with H1299-control, the expression of TMEM106A in cell line H1299-TMEM106A was significantly increased. The proliferation experiment showed that the high expression of TMEM106A significantly inhibited the proliferation of lung cancer cells. The colony formation assay showed that the colony forming ability of lung cancer cells with high expression of TMEM106A in cell line H1299-TMEM106A was significantly lower than that of H1299-control lung cancer cells (P<0.01). CONCLUSION: TMEM106A can inhibit the proliferation of lung cancer cells.

Key words: lung cancer, TMEM106A