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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (6): 638-641.

• 研究原著 • 上一篇    下一篇

复方积雪草对局灶硬化性肾小球肾炎模型小鼠肾组织内细胞因子表达的调控作用

王军, 程晓霞, 杨汝春, 朱晓玲, 鲁盈, 王永钧   

  1. 浙江中医学院附属第二医院肾病实验室,杭州 310007,浙江
  • 收稿日期:2003-06-30 修回日期:2003-07-10 出版日期:2003-12-26 发布日期:2020-11-19
  • 通讯作者: 王军,男,医学硕士,主管技师,研究方向:主要从事肾脏病药理学和免疫学研究。Tel:0571-85157591-5565 E-mail:zhaohen0908@163.com
  • 基金资助:
    杭州市科委重大项目资金项目(No2001113A09)

Modification of Centella asiatica compound on renal cytokines expressionProfiles in mice models with focal glomerulosclerosis

WANG Jun, CHEN Xiao-Xia, YANG Ru-Chun, LU Ying, WANG Yong-Jong   

  1. Laboratory of Nephrology,the Second Affiliated Hospital,Zhejiang Traditional Medical College,Hangzhou 31007,Zhejiang,China
  • Received:2003-06-30 Revised:2003-07-10 Online:2003-12-26 Published:2020-11-19

摘要: 目的 了解局灶硬化性肾小球肾炎(FGS)模型小鼠的细胞因子表达谱,以及复方积雪草对其细胞因子网络的表达调控作用。方法 通过BALB/c 小鼠尾静脉一次性注射阿霉素(11 mg·kg-1),诱导成FGS 肾炎模型,以复方积雪草制剂灌胃治疗4 wk,分别取1 、4 wk 肾组织,提取其总RNA,通过反转录掺入荧光素CY3-dUTP/CY5-dUTP,转录产物与细胞因子基因芯片杂交,芯片扫描并进行图像分析。结果 基因表达谱芯片检测表明:复方积雪草可抑制PDGF-α、PDGF-β 、PDGF 受体、VEGF mRNA 的表达;能抑制IL-9 、IL-7R2 、MIP等因子的mRNA 的表达;经复方积雪草刺激后,肾组织中癌基因c-Myc,Jun 表达被抑制。结论 复方积雪草具有抗肾纤维化作用,其作用效应可能是多种细胞因子共同调控的结果。

关键词: 药理学, 复方积雪草, 细胞因子, 基因芯片, 中药, 肾小球硬化

Abstract: AIM: To study the cytokine gene expressionProfile in mice model with focal glomerulosclerosis(FGS)nephritis and the mechanism of Centella asiatica compound on cytokines network.METHODS: FGS model mice of BALB/c were induced by adromycin(ADR,11mg·kg-1),and then treated with Centella asiatica compound for four weeks.The values of total RNA werePrepared from the kidney tissue at the end of first week and fourth week.The cDNA retained by the reversal react andlabeled with CY3-dUTP/CY5-dUTPin fluorescence,hybridized with the cytokines genes microarray.The chips were scanned in SCANARRAY 3000 and the images were analyzed by Image 4.0 software.RESULTS: The expression ofPDGF-α,PDGF-β,PDGF-R and VEGF mRNA were down-regulated after treated by Centella asiatica compound ;the expressions of IL-9,IL-7R2,and MIPwere down-regulated after treated by the compound.At the same time,through the stimulation of the compound,the expressions of c-Myc and Jun in kidney were restrained.CONCLUSION: Centella asiatica compound has a function of anti-glomerulosclerosis,and it may be a result of various cytokines worked together.

Key words: pharmacology, Centella asiatica compound, cytokine, DNA chip, TCM, glomerulosclerosis

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