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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1154-1156.

• 研究原著 • 上一篇    下一篇

大蒜黄酮的分离及抗内毒素活性的评价

蒋栋能, 郑江   

  1. 第三军医大学附属西南医院综合实验研究中心, 重庆400038
  • 收稿日期:2004-07-28 修回日期:2004-08-26 出版日期:2004-10-26 发布日期:2020-11-23
  • 通讯作者: 郑江, 男, 副研究员, 硕士导师, 主要从事拮抗脓毒症药物的设计和筛选。
  • 作者简介:蒋栋能, 男, 硕士, 主管技师。Tel:023-68765466 Email:jiangdn @mail.tmmu. com. cn

Extraction of garlic flavone and its antiendotoxin activity

JIANG Dong-Neng, ZHENG Jiang   

  1. Medical Research Center, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China
  • Received:2004-07-28 Revised:2004-08-26 Online:2004-10-26 Published:2020-11-23

摘要: 目的: 筛选和评价大蒜提取成分的抗内毒素作用。方法: 通过离子交换-高效液相色谱等方法从大蒜中筛选出2 个成分(大蒜黄酮1 、2), 采用动态浊度法测定其体外中和内毒素的能力, 观察其对内毒素(20 mg·kg-1)攻击小鼠的保护作用。结果: 大蒜黄酮2 能直接中和内毒素, 对内毒素攻击小鼠有显著的保护作用。结论: 大蒜黄酮2 具有显著的抗内毒素活性。

关键词: 大蒜, 黄酮, 抗内毒素, 活性成分

Abstract: AIM: To extract garlic flavone and evaluate its antiendotoxin activity.METHODS: The two fractions (garlic flavone 1, garlic flavone 2)were screened and extracted by ion exchange-HPLC, and the neutralization ability of the garlic flavone to lipopolysaccharide (LPS)was determined by kinetic turbidimetric assay. The protective effect of the garlic flavone 2 was observed in mice attacked by 20 mg·kg-1 of LPS.RESULTS: The garlic flavone 2 could neutralise LPS and protect mice from the attack of LPS.CONCLUSION: The garlic f lavone 2 has a significant antiendotoxin activity on LPS-attacked mice.

Key words: garlic, flavone, antiendotoxin, active fraction

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