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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (3): 337-339.

• 研究原著 • 上一篇    下一篇

咖啡酸、阿魏酸和香草酸对酪氨酸酶活性的影响

闫军, 李昌生, 陈声利1, 张君仁2   

  1. 济南市皮肤病防治院药剂科, 济南250001, 山东;
    1山东省皮肤病性病研究所皮肤科, 济南250022, 山东;
    2山东大学药学院药物分析教研室, 济南250012, 山东
  • 收稿日期:2003-10-13 修回日期:2003-11-28 出版日期:2004-03-26 发布日期:2020-11-21
  • 通讯作者: 闫军, 男, 主管药师, 研究方向:临床药学及制剂开发。Tel:0531-7931386-8008  E-mail:yjcsl 123@hotmail.com

Effects of caffeic acid, ferulic acid and vanillic acid on tyrosinase

YAN Jun, LI Chang-Sheng, CHEN Sheng-Li1, ZHANG Jun-Ren2   

  1. Department of Pharmacy, Jinan Hospital for Skin Diseases Prevention, Jinan 250001, Shandong, China;
    1Department of Dermatology, Shandong Provincial Institute of Dermatology and Venereology, J inan 250022, Shandong, China;
    2Department of Pharmaceutical Analysis, School of Pharmacy, Shandong University, Jinan 250012, Shandong, China
  • Received:2003-10-13 Revised:2003-11-28 Online:2004-03-26 Published:2020-11-21

摘要: 目的:研究咖啡酸、阿魏酸和香草酸对酪氨酸酶活性的影响, 寻找酪氨酸酶抑制剂, 从而为治疗色素增加性皮肤病筛选药物。方法:酪氨酸酶多巴速率氧化法体外测定药物干预前后酪氨酸酶活性, 求出酪氨酸酶抑制率。采用Lineweaver-Burk 双倒数法制得酶动力学曲线, 推断抑制类型。结果:3 种试药中仅香草酸对酪氨酸酶具有抑制作用, 与氢醌比较无显著差异(P >0.05), 咖啡酸、阿魏酸对酪氨酸酶具有上调激活作用。结论:香草酸为良好的混合型酪氨酸酶抑制剂。

关键词: 咖啡酸, 阿魏酸, 香草酸, 酪氨酸酶, 抑制剂

Abstract: AIM: To evaluate the effects of monomers of caffeic acid, ferulic acid and vanillic acid on the activity of tyrosinase, so as to provide a kind of drug in the treatment of hyperpigmentation.METHODS: Tyrosinase activity was estimated by measuring the oxidation rate of L-DOPA.The inhibitory pattern of each monomer was determined according to their Lineweaver-Burk curve compared to the controlled group.RESULTS: Vanillic acid showed an inhibition to tyrosinase activity, and the rate of inhibitory was similar to hydroquinone, which had no statistically significant difference between them (P >0.05). Caffeic acid and ferulic acid showed an up-regulation to tyrosinase activity.CONCLUSION: Vanillic acid, a mixed-type inhibitor of tyrosinase, can inhibit the activity of tyrosinase greatly.

Key words: caffeic acid, ferulic acid, vanillic acid, tyrosinase, inhibitor

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