无创性延迟肢体缺血预适应对糖尿病大鼠心肌缺血/再灌注氧化损伤的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (11): 1220-1225.

无创性延迟肢体缺血预适应对糖尿病大鼠心肌缺血/再灌注氧化损伤的影响

李玉梅¹, 朱学慧^1,2, 袁恒杰¹, 罗琼¹, 吴艳娜¹, 康毅¹, 焦建杰¹, 高卫真¹, 刘艳霞¹, 娄建石¹

¹天津医科大学基础医学院药理学教研室,²药学院临床药学教研室, 天津 300070

收稿日期:2008-08-25 修回日期:2008-11-08 出版日期:2008-11-26 发布日期:2020-10-14
通讯作者: 娄建石,男, 教授, 博士生导师, 研究方向:心血管药理学。Tel:13920412457 E-mail: jianshilou@sina.com
作者简介:李玉梅, 女, 硕士研究生, 研究方向:心血管药理学。Tel:13920978302 E-mail: lym-1201@sina.com
基金资助:
天津市自然科学基金资助项目(003608411)

Effects of noninvasive delayed limb ischemic preconditioning on oxidative injury following myocardial ischemia /reperfusion in diabetic rats

LI Yu-mei¹, ZHU Xue-hui^1,2, YUAN Heng-jie¹, LUO Qiong¹, WU Yan-na¹, KAN Yi¹, JIAO Jianjie¹, GAO Wei-zhen¹, LIU Yan-xia¹, LOU Jian-shi¹

¹Department of Pharmacology of Basic Medical College,²Department of Clinical Pharmacy of College of Pharmacy, Tianjin Medical University, Tianjin 300070, China

Received:2008-08-25 Revised:2008-11-08 Online:2008-11-26 Published:2020-10-14

摘要/Abstract

摘要： 目的: 观察无创性延迟肢体缺血预适应(NDLIP) 对糖尿病(DM) 大鼠心肌缺血/再灌注氧化损伤的保护作用。方法: 尾静脉注射链脲佐菌素(STZ) 制备DM 大鼠模型。将DM 大鼠随机分成心肌缺血再灌注(I/R)、心肌缺血预适应(MIP)、无创性延迟肢体缺血预适应(NDLIP) 组。通过3个循环的左后肢5 min 缺血 /5 min 再灌注, 每天1次, 连续3 d, 建立NDLIP 模型。心肌冠状动脉左前降支(LAD) 实施3 次5 min 缺血 /5 min 再灌注建立MIP 模型。各组实施LAD 30 min 缺血 /120 min 再灌注复制I/R 模型。用BL-420E 生物机能实验系统连续监测心电图(ECG), 记录缺血期间室性心律失常(VA) 的发生情况。TTC 染色测定大鼠心肌I/R 后梗死面积(IS) 。检测心肌组织中总-超氧化物歧化酶(T-SOD)、锰-超氧化物歧化酶(Mn-SOD) 活性及丙二醛(MDA) 含量。结果: 与I/R 组相比, MIP 组和NDLIP 组室性早搏(VPC)出现时间明显推迟(P<0.01), 持续时间明显缩短(P<0.01), 室性心动过速(VT) 和心室纤颤(VF) 发生率都明显降低(P<0.05), IS 明显缩小,梗死面积/危险区(IS /AAR) 重量比值显著降低(P<0.01), 心肌组织MDA 含量均明显下降(P<0.01), 而T-SOD 及Mn-SOD 活性均明显升高(P<0.01) 。结论: 无创性延迟肢体缺血预适应可以改善DM 大鼠的心肌抗氧化能力。

关键词: 肢体, 远端缺血预适应, 糖尿病, 缺血/再灌注损伤, 抗氧化作用, 心脏保护

Abstract: AIM: To study the protection of noninvasive delayed limb ischemic preconditioning (NDLIP) on myocardial ischemia/reperfusion oxidative injury in diabetic rats. METHODS: Diabetic rat models were induced by injecting streptozotocin (STZ) into the vena caudalis. The diabetic rats were divided randomly into three groups: myocardial ischemia/reperfusion (I/R) group, myocardial ischemic preconditioning (MIP) group and noninvasive delayed limb ischemia preconditioning (NDLIP) group. The rats were pretreated with three cycles of 5 min ischemia /5 min reperfusion on the left hind limb, once a day for three consecutive days, to establish the NDLIP models. And MIP models were subjected to three episodes of 5 min of ischemia coupled to 5 min of reperfusion in the left anterior descending (LAD) coronary artery of rats. The models of I/R were established by 30 min ischemia and 120 min reperfusion of LAD in rats. The electrocardiogram was monitored continuously to record ventricular arrhythmia (VA) during 30 min ischemia. I/R-induced infarct size (IS) was determined using triphenyltetrazolium chloride (TTC) staining. Myocardial total superoxide dismutase (T-SOD) and manganese superoxide dismutase (Mn-SOD) activity, and malondialdehyde (MDA) content were determined respectively in each group rats. RESULTS: Compared with group I/R, the onset of ventricular premature contraction (VPC) was delayed markedly (P<0.01), duration of VPC was shortened (P<0.01), incidences of ventricular tachycardia (VT) and ventricular fibrillation (VF) were decreased (P<0.01), myocardial IS was diminished (P<0.01), infarct size /area at risk(IS AAR) was degraded, myocardial MDA content was declined (P<0.01), myocardial T-SOD and Mn-SOD activity were increased (P<0.01) in group MIP, group NDLIP.CONCLUSION: Noninvasive delayed limb ischemic preconditioning can improve the myocardial antioxidation of diabetic rats.

Key words: limb, remote ischemic preconditioning, diabetes mellitus, ischemia /reperfusion injury, antioxidation, heart protection

中图分类号:

R965.2

李玉梅, 朱学慧, 袁恒杰, 罗琼, 吴艳娜, 康毅, 焦建杰, 高卫真, 刘艳霞, 娄建石. 无创性延迟肢体缺血预适应对糖尿病大鼠心肌缺血/再灌注氧化损伤的影响[J]. 中国临床药理学与治疗学, 2008, 13(11): 1220-1225.

LI Yu-mei, ZHU Xue-hui, YUAN Heng-jie, LUO Qiong, WU Yan-na, KAN Yi, JIAO Jianjie, GAO Wei-zhen, LIU Yan-xia, LOU Jian-shi. Effects of noninvasive delayed limb ischemic preconditioning on oxidative injury following myocardial ischemia /reperfusion in diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(11): 1220-1225.

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