果糖二磷酸锶改善糖尿病大鼠睾丸病变及对前原内皮素和内皮素转变酶表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (12): 1339-1343.

果糖二磷酸锶改善糖尿病大鼠睾丸病变及对前原内皮素和内皮素转变酶表达的影响

张琪¹, 汤晓赟², 戴德哉², 应汉杰¹, 戴茵²

¹南京工业大学制药与生命科学学院,
²中国药科大学药理研究室, 南京 210009, 江苏

收稿日期:2008-07-05 修回日期:2008-08-01 发布日期:2020-10-30
通讯作者: 戴德哉,男,教授,研究方向:心血管药理学、糖尿病药理学、男性性功能药理学。Tel:025-83271270E-mail:dezaidai@vip.sina.com
作者简介:张琪,女,副教授,主任,药理学博士,研究方向:药理学,药物动力学。Tel:025-83285206 E-mail:zqapple@126.com
基金资助:
国家自然科学基金项目(30572217)

Effects of strontium fructose 1, 6-diphosphate (FDP-Sr) on didymus lesion and the mRNA expressions of prepro ET-1(PPET-1) and endothelin converting enzyme(ECE) in diabetic rats

ZHANG Qi¹, TANG Xiao-yun², DAI De-Zai², YING Han-jie¹, DAI Yin²

¹College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology,
²Research Division of Pharmacology, China Pharmaceutical University, Nanjing 210009, Jiangsu, China

Received:2008-07-05 Revised:2008-08-01 Published:2020-10-30

摘要/Abstract

摘要： 目的研究果糖二磷酸锶(FDP-Sr) 对糖尿病睾丸病变的改善, 与丙酸睾酮对比, 并初步探讨其可能机制。方法链脲佐菌素致糖尿病雄性大鼠8 周, 在后4 周, 分别每日灌胃FDP-Sr(50, 100,200 mg/kg) 以及丙酸睾酮(4 mg/kg, s.c.治疗),测血中睾酮、谷胱甘肽过氧物酶(GSHpx) 、睾丸中内皮素-1(ET-1) 系统中前原ET-1(前原内皮素,ppET-1) 和ET 转变酶(ECE) 的mRNA 表达, 观察睾丸组织学改变。结果糖尿病大鼠睾丸曲细精管萎缩明显, 血中睾酮、GSHpx 明显降低, ppET-1和ECE 呈高表达。上述改变被FDP-Sr 明显改善,丙酸睾酮作用不明显。结论 FDP-Sr 治疗糖尿病睾丸病变优于丙酸睾酮, 可能与抑制睾丸中内皮素系统的高表达相关。

关键词: 糖尿病, 睾丸病, 内皮素, 果糖二磷酸锶, 睾酮, 替代治疗

Abstract: AIM: To determine the benefits of FDP-Sr on streptozotocin (STZ) induced diabetic testopathy in rats, compared with testosterone propionate, and explore its likely mechanisms.METHODS: Male SD rats were given streptozotocin to induce strontium fructose 1, 6-diphosphate (EDP-sr) diabetes for 8 weeks and in the second 4 weeks rats were treated with FDP-Sr (50, 100, 200 mg/kg, i.g.) and testosterone propionate(4 mg/kg, s.c.).GSH-px, mRNA expression of perpro ET-1 (ppET-1), endothelin coverting enzyme(ECE) in testicular tissues, morphological changes and blood testosterone were investigated.RESULTS: Seminiferous tubules in diabetic rats were significantly atrophied relative to the normal.The expressions of ppET-1 and ECE were upregulated significantly in association with decreased serum testosterone and decreased GSH-px significantly.FDP-Sr attenuated these alterations significantly, but testosterone propionate was less effective.CONCLUSION: FDP-Sr relieves diabetes induced male hypogonadism in rats in superior to that of testosterone propionate.It is likely to be related by suppressing over-active endothelin system in the testis.

Key words: diabetes, testopathy, endothelin, strontium fructose 1, 6-diphosphate, testosterone, supplement therapy

中图分类号:

R587.1

张琪, 汤晓赟, 戴德哉, 应汉杰, 戴茵. 果糖二磷酸锶改善糖尿病大鼠睾丸病变及对前原内皮素和内皮素转变酶表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(12): 1339-1343.

ZHANG Qi, TANG Xiao-yun, DAI De-Zai, YING Han-jie, DAI Yin. Effects of strontium fructose 1, 6-diphosphate (FDP-Sr) on didymus lesion and the mRNA expressions of prepro ET-1(PPET-1) and endothelin converting enzyme(ECE) in diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1339-1343.

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