依那普利对大鼠离体胸主动脉的舒张作用及其机制

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (2): 149-153.

依那普利对大鼠离体胸主动脉的舒张作用及其机制

王丹¹, 张轩萍¹, 董秋菊², 张明升¹, 牛龙刚¹, 常海良¹

¹山西医科大学药理教研室, ²山西医科大学第一附属医学院心血管内科, 太原 030001, 山西

收稿日期:2007-11-19 修回日期:2008-01-23 出版日期:2008-02-26 发布日期:2020-10-14
通讯作者: 张轩萍, 女, 博士, 副教授, 硕士生导师, 研究方向:心血管药理学。Tel:0351-4135079-8001 E-mail:zxp71122@yahoo.com.cn
作者简介:王丹, 女, 硕士研究生, 研究方向:心血管药理学。Tel:0351-4135079-8006 E-mail:wangdadan7880@sina.com
基金资助:
山西省青年基金资助项目(20041045)

Vasodilative effect and mechanism of enalapril on thoracic aorta of rats

WANG Dan¹, ZHANG Xuan-ping¹, DONG Qiu-ju², ZHANG Ming-sheng¹, NIU Long-gang¹, CHANG Hai-liang¹

¹Department of Pharmacology, Shanxi Medical University, ²Department of Cardiology, First Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi, China

Received:2007-11-19 Revised:2008-01-23 Online:2008-02-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:观察依那普利对血管的直接作用, 并探讨其机制。方法:采用Powerlab 生物信号采集系统记录依那普利对去甲肾上腺素(NE) 和KCl预收缩的离体大鼠胸主动脉环舒张作用, 观察左旋硝基精氨酸甲酯(L-NAME, 10^-4 mol/L) 和吲哚美辛(10^-8 mol/L) 对其作用的影响。结果:在内皮完整的大鼠离体胸主动脉环, 依那普利(10^-9~10^-4 mol/L) 对NE(10^-5 mol/L) 或KCl (20 mmol/L)引起的收缩具有浓度依赖性的舒张作用。去内皮后, 依那普利的舒血管作用显著减弱。在内皮完整的血管环, L-NAME (10^-4mol/L) 和吲哚美辛(10^-8 mol/L) 对依那普利的舒血管作用具有明显的抑制作用。结论:依那普利对大鼠离体胸主动脉环具有浓度依赖性的舒张作用, 此作用具有内皮依赖性, 与内皮产生的NO 和前列环素(PGI₂)有关。

关键词: 依那普利, 胸主动脉, 血管舒张, 内皮, 一氧化氮, 前列环素

Abstract: AIM:To investigate the vasodilative effects and the possible mechanism of enalapril on thoracic aorta of rats. METHODS:Isotonic tension of thoracic aortic rings precontracted by norepinephrine (NE, 10^-5 mol/L) or KCl (20mmol/L) was recorded. The vascular tension was recorded by Powerlab multichannel physiology signal acquisition and analysis computer system by tension transducers. The vasorelaxing action of enalapril and effects of various drugs which include NG-nitro-L-arginine methyl ester (10^-4mol/L) and indomethacin (10^-8 mol/L) were observed in the rings with endothelium intact or denuded. RESULTS:Enalapril (10^-9 -10^-4mol/L) induced concentrationdependent relaxation in thoracic aortas with intact endothelium. There was significant difference between the rings with intact and denuded endothelium. N^G-nitro- L-arginine methyl ester (10^-4mol/L) and indomethacin (10^-8mol/L) can significant inhibit the relaxtion effects of enalapril on thoracic aortic rings. CONCLUSION:We conclude that enalapril preferentially antagonized the contraction of rat thoracic artery in a concentration-dependent and endothelium-dependent manner. The vasorelaxation of enalapril is relevant to the production of nitric oxide and prostacyclin.

Key words: enalapril, thoracic aorta, vasodilation, endothelium, nitric oxide, prostacyclin

中图分类号:

R965.2

王丹, 张轩萍, 董秋菊, 张明升, 牛龙刚, 常海良. 依那普利对大鼠离体胸主动脉的舒张作用及其机制[J]. 中国临床药理学与治疗学, 2008, 13(2): 149-153.

WANG Dan, ZHANG Xuan-ping, DONG Qiu-ju, ZHANG Ming-sheng, NIU Long-gang, CHANG Hai-liang. Vasodilative effect and mechanism of enalapril on thoracic aorta of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(2): 149-153.

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