关键词: 多肽ZS-1, cDNA文库, 减性筛选, 肺癌, 肿瘤标志物

Abstract: AIM: To select a potential biomarker for early lung cancer diagnosis and targeted therapy by using the cancer specific bounded peptide ZS-1 which had already been obtained from the laboratory.METHODS: The peptide ZS-1 marked by biotin was used as a probe to pan out the human lung cancer cDNA phage display library,after 3 rounds of subtraction panning,the specific binding clones of ZS-1 were identified.After amplification and purification,then those DNA sequences were identified and analyzed with bioinformatics. Tissue microarray technology was used to detect biomelecule in tissues.RESULTS: The lung cancer membrane receptor protein KIAA1199 binding ZS-1 was first found by bioinformatics analysis. Subsequently, a specific human lung cancer tissue microarray was detected and analysed by KIAA199 antibody.The results displayed KIAA1199 positive detection rate was 32.8%.CONCLUSION: A tumor biomarker KIAA1199 selected from human lung cancer cDNA library provides a potential tool for early lung cancer diagnosis and therapy.

Key words: Peptide ZS-1, cDNA library, Subtract bio-panning, Lung cancer, Tumor markers