新型超顺磁性氧化铁在干细胞活体示踪中的应用研究

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (3): 272-276.

新型超顺磁性氧化铁在干细胞活体示踪中的应用研究

谢青松¹, 许信龙¹, 魏晓捷¹, 潘红松¹, 傅小君¹, 陈再丰¹, 李立新²

¹慈溪市人民医院神经外科, 慈溪315300, 浙江;
²南京医科大学第一附属医院神经外科, 南京210029, 江苏

收稿日期:2009-12-29 修回日期:2009-12-29 发布日期:2020-10-14
通讯作者: 李立新, 男, 博士, 教授, 博士生导师, 研究方向:中枢神经损伤与修复。E-mail:lilixin2@hotmail.com
基金资助:
宁波市自然科学基金资助项目(2008A610093); 国家自然科学基金资助项目(30973092)

Application research of superparamagnetic iron oxide in vivo tracing of stem cells

XIE Qing-song¹, XU Xin-long¹, WEI Xiao-jie¹, PAN Hong-song¹, FU Xiao-jun¹, CHEN Zai-feng¹, LI Li-xin²

¹Department of Neurosurgery, People's Hospi tal of Cixi City, Cixi 315300, Zhejiang, China;
²Department of Neurosurgery, the First Affiliated Hospital, Nanjing Medical University, Nanjing 10029, Jiangsu, China

Received:2009-12-29 Revised:2009-12-29 Published:2020-10-14

摘要/Abstract

摘要： 目的: 研究超顺磁性氧化铁(superparamagne tic i ron o xide, SPIO) 标记干细胞的效率及其在磁共振活体示踪中的应用价值。方法: 以3-氨丙基三乙氧基硅烷(APTS) 修饰Fe2O3 配制成S PIO, 用SPIO 转染标记骨髓间充质干细胞(MSCs), 对标记后的MSCs 行普鲁士蓝染色鉴定、MTT 测试细胞相对数量。最后将标记后的MSCs 移植入大鼠脑内, 磁共振(MRI) 扫描示踪显示。结果: 普鲁士蓝染色可直接显示SPIO 高效率标记MSCs, MT T 测试表明SPIO 标记对MSC s 增殖数量及其活力无明显影响, MRI 检查发现脑内移植的SPIO 标记MSCs 在T2 上呈明显的低信号。结论: APTS 修饰Fe2O3 配制而成的新型SPIO 对细胞无明显毒性, 没有多聚赖氨酸(PLL) 等转染剂仍可直接高效率标记MSCs,MRI 活体显影示踪效果良好。

关键词: 超顺磁性氧化铁, 干细胞, 磁共振成像

Abstract: AIM: To investing ate a new superparamagnetic iron oxide(SPIO) which could label stem cell seff ciently and be traced in vivo by magnetic resonance with out poly-L-ly sine or othertrans fection agent. METHODS: SPIO nano particles was prepared with Fe2O3 coated by athin layer of 3-amino pro py ltriethoxy silane, and then the compound (SPIO) was used to labeled Marrow Mesenchymal Stem Cells (MSCs).The labeled MSCs were identif icated by prussian blue stain and counted by MTT. Atlast, labeled MSCs was transplanted into rat brain and scanned by MRI. RESULTS: Prussian blue stain indicated that MSCs could be high efficiently labeled by SPIO, and there was no significant effect on the proliferation and viability of labeled MSCs by MTT. Hypo-intense changes of MRI T2 examination could be o bserved in the transplanted area of MSCs labeled by SPIO after t ransplantation in rats brain. CONCLUSION: The new SPIO(Fe2O3 coated by a thin layer of 3-aminopropy ltrie thoxy silane) had no significant toxicity on cells. MSCs still could be label led with the new SPIO directly and high efficiently without the use of poly-L-lysine or other additional transfecting agents. The transplanted cells could be well imaged and traced in vivo.ysine or other additional t ransfecting agents. The transplanted cells could be well imaged and t raced in vivo.

Key words: Superparamagneticiron oxide, Stem cell, Mag netic resonance imaging

中图分类号:

R965.2

谢青松, 许信龙, 魏晓捷, 潘红松, 傅小君, 陈再丰, 李立新. 新型超顺磁性氧化铁在干细胞活体示踪中的应用研究[J]. 中国临床药理学与治疗学, 2010, 15(3): 272-276.

XIE Qing-song, XU Xin-long, WEI Xiao-jie, PAN Hong-song, FU Xiao-jun, CHEN Zai-feng, LI Li-xin. Application research of superparamagnetic iron oxide in vivo tracing of stem cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(3): 272-276.

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