以 Lipid A 为靶点拮抗内毒素中药的筛选

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (12): 1349-1353.

以 Lipid A 为靶点拮抗内毒素中药的筛选

陈益国, 姚婕, 张向武, 蒋栋能, 郭毅斌, 魏利召, 程娟, 吴羽中, 郑江

第三军医大学西南医院综合实验研究中心, 重庆 400038

收稿日期:2005-09-02 修回日期:2005-10-10 出版日期:2005-12-26 发布日期:2020-11-11
作者简介:陈益国,男,硕士,讲师,研究方向:主要从事拮抗脓毒症天然药物的筛选和分离纯化。Tel:(0)13101292035E-mail:qjkr2@tom.com。

Screening of anti-endotoxin traditional Chinese medicine by target of Lipid A

CHEN Yi-guo, YAO Jie, ZHANG Xiang-wu, JIANG Dong-neng, GUO Yi-bin, WEI Li-zhao, CHENG Juan, WU Chong, ZHENG Jiang

Juan,WU Chong,ZHENG JiangMedical Research Center,Southwestern Hospital,Third Military Medical University,Chongqing 400038,China

Received:2005-09-02 Revised:2005-10-10 Online:2005-12-26 Published:2020-11-11

摘要/Abstract

摘要： 目的: 利用生物传感器跟踪技术从抗炎中药中筛选出具有拮抗内毒素的中药,为进一步对中药拮抗内毒素活性成分的分离纯化提供实验依据。方法: 将革兰阴性细菌的脂质 A(Lipid A) 包被于生物传感器的非衍生板建立靶点,跟踪测定赤芍、大黄、黄芩等 78 种中药的水提物和醇提物与 Lipid A 的结合活性,再将筛选出的中药去鞣质后与定量的内毒素(LPS,20、50 ng·ml^-1) 37 ℃孵育 30 min 后,测定孵育后的样品与 Lipid A 的结合情况,以评价样品内的活性物质含量。结果: 在所筛选的78 种中药中有12种中药的水提物与 Lipid A 具有较高的亲和活性,在这12 种中药中有 6 种中药的醇提物与 Lipid A 也具有较高的亲和活性;通过对所筛选出的 12 种中药的水提物去除鞣质后与 LPS 的消耗实验,发现所筛选出的 12 种中药均含有除鞣质以外的与 lipid A 具有特异性结合的物质,并且 12 种中药中能与 Lipid A发生特异性结合作用的活性物质含量存在较大的差异。结论: 生物传感器跟踪技术是一种快速、准确、有效的筛选平台,筛选出的 12 种中药均含有非鞣质类能与 lipid A 发生特异性结合作用的物质,为进一步对 12种中药抗内毒素有效部位或单体的分离提供了重要的实验依据。

关键词: 内毒素, 脂质A, 生物传感器, 中药, 筛选

Abstract: AIM: To screen anti-endotoxin traditional Chinese medicine(TCM) from anti-inflammation TCM by Biosensor,and to furtherstudy anti-endotoxin effective materials of TCM.METHODS: Non-derivatised cuvette immobilised Lipid A was used to track the bonding be-tween the Lipid A and aqueous extracts and alcoholic ex-tracts from seventy-eight TCM,and then neutralized endo-toxin in vitro by selecting hypso-bonding TCM that re-moved the tannins.RESULTS: Twelve aqueous extracts and six alcoholic extracts from seventy-eight TCM had hypso-bonding with Lipid A (more than 200 response unit),and twelve aqueous extracts could bind specially endotoxin afterremoved tannins.CONCLUSION: Track of biosensortechnology is a quick,accurate and valid method in screening TCM.Aqueous extracts of twelve TCM can bind lipid A specially besides the tannins,and it is foundation to furthersegregate anti-endotoxin effective materials from twelve TCM in the future.

Key words: endotoxi, lipid A, biosensor, TCM, screen

中图分类号:

R285.5

陈益国, 姚婕, 张向武, 蒋栋能, 郭毅斌, 魏利召, 程娟, 吴羽中, 郑江. 以 Lipid A 为靶点拮抗内毒素中药的筛选[J]. 中国临床药理学与治疗学, 2005, 10(12): 1349-1353.

CHEN Yi-guo, YAO Jie, ZHANG Xiang-wu, JIANG Dong-neng, GUO Yi-bin, WEI Li-zhao, CHENG Juan, WU Chong, ZHENG Jiang. Screening of anti-endotoxin traditional Chinese medicine by target of Lipid A[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(12): 1349-1353.

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