Abstract:

AIM: To investigate the mechanism and the effects of Rufuning granules on improving the symptom of mammary gland hyperplasia（MGH）． METHODS: Forty-eight rabbits were randomly divided into six groups (n=8), normal control group, model group, Rupixiao tablets group, Rufuning-high dose, medium dose and low dose groups. The model of MGH was induced by injecting estradiol benzoate in unpregnant female rabbits. Rabbits in the normal control received saline injection only. The breast diameter and nipple height of rabbits were determined. The contents of prolactin（PRL）, estradiol（E2）and progesterone（P）in serum in each group were detected．Pathologic changes in morphology due to MGH in rabbits were observed by microscopy. Meanwhile, the expression of VEGF protein in rabbit breast tissue was determined. RESULTS: The breast diameter and nipple height of rabbits were reduced after treatment in high and medium doses of Rufuning granules group（P<0.05）．The contents of PRL and E2 in serum were reduced（P<0.05）while those of progesterone in serum were improved（P<0.05）．The performance of histology breast hyperplasia was significantly improved. The number of mammary gland flocculus and lobular gland bubble and lumen expansion were reduced in rabbit mammary. Mammary gland hyperplasia of interstitial fibrous connective tissue was also significantly alleviated as compared with those in model group．Meanwhile, VEGF over-expression in breast tissue was reduced. CONCLUSION: Rufuning granules may improve symptom of MGH by adjusting the ratio of estrogen and progesterone and reducing VEGF over-expression．

Key words: Rufuning granules, mammary gland hyperplasia, estradiol, progesterone, VEGF