Expression trend of autophagy in mice with renal interstitial fibrosis

doi:10.12092/j.issn.1009-2501.2019.03.004

Abstract

Abstract:

AIM: To observe the expression trend of autophagy at different time points after UUO surgery. METHODS: Thirty-two clean healthy male ICR mice were randomized into Sham operation (Sham) group and UUO model (UUO) group, and mice were sacrificed at 3, 7 and 14 d after UUO surgery, respectively, 8 mice for each group. Serum was collected to detect Scr, BUN, HE and Masson staining ways were applied to the renal tissue, and the formation of autophagosomes was observed by electron microscopy and the expression of LC3 protein and P62 protein in renal tissue was detected by Westernblot. RESULTS:The renal cortex thinned, tubule atrophy, interstitial fibrosis, single nuclear cell infiltration, no obvious glomerular lesions were found in the obstructed side of UUO postoperatively, and the pathological changes were most prominent on 14 d after UUO surgery. Compared with the Sham group, LC3-Ⅱbegan from 3 d, peaked on 7 d, down on 14 d; P62 was different, 3 d began to decline, 7 d reached the lowest, 14 d recovered gradually, which showed that autophagy was most significant at 7 d. Electron microscopy showed that autophagosome and phagocytic vacuoles appeared 7 days after UUO surgery.CONCLUSION: The expression of autophagy after UUO surgery had a certain time dependence, which was most significant at 7 d, and the severity of autophagy was not parallel to the pathological severity.

Key words: UUO, autophagy, LC3-Ⅱ, P62

CLC Number:

R965.2

CHEN Xinxin, CHEN Yu, ZHENG Chenfei, ZHOU Ying, CHEN Chaosheng. Expression trend of autophagy in mice with renal interstitial fibrosis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(3): 260-265.

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