Experimental study on the effect of curcumin on endometriosis in rats

doi:10.12092/j.issn.1009-2501.2020.08.004

Abstract

Abstract: AIM: To analyze the effect of curcumin on the expression of transcription factors Twist1, Twist2, E-cadherin and Syndecan-1 in rats with endometriosis. METHODS: Thirty SD rats were randomly divided into five groups: model group, low dose curcumin group (25 mg·kg-1·d-1), medium dose curcumin group (50 mg·kg-1·d-1), high dose curcumin group (100 mg·kg-1·d-1) and gestrinone group (0.5 mg·kg-1·d-1). There were 6 rats in each group, and 5 rats in sham operation group were set up as blank control group. The expression of Twist1, Twist2, E-cadherin and Syndecan-1 was detected by immunohistochemistry. RESULTS: Compared with the model group, the ectopic tissue volume of curcumin group and gestrinone group was significantly lower, the difference was statistically significant (P<0.05). Positive Twist1 was mainly located in the nucleus, positive Twist2 was mainly located in the cytoplasm, positive E-cadherin and Syndecan-1 staining was mainly located in the epithelial cell membrane, rarely in individual cytoplasm. Compared with the model group, the scores of Twist1, Twist2 and Syndecan-1 in each dose group of curcumin and gestrinone group were significantly lower, and the scores of Twist1, Twist2 and Syndecan-1 in low and middle dose curcumin group were significantly higher than those in gestrinone group, and the scores of E-cadherin were lower than those in gestrinone group (P<0.05). The scores of Twist1, Twist2, E-cadherin and Syndecan-1 in high dose curcumin group were significantly higher. There was no significant difference between the high dose curcumin group and gestrinone group (P>0.05). CONCLUSION: Curcumin can reduce the expression of Twist1, Twist2 and Syndecan-1, increase the expression of E-cadherin, and inhibit the growth of endometrium in the model rats, so as to achieve the purpose of treatment.

Key words: curcumin, endometriosis, Twist1, Twist2, E-cadherin, Syndecan-1

CLC Number:

SHEN Longde, LI Ailu, LI Lichun, WU Yinyin, SHEN Longyuan, JIANG Qiuxia, HE Jinxi, WU Zhifen, YU Liji, ZHANG Xiaohong. Experimental study on the effect of curcumin on endometriosis in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(8): 862-868.

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