Effects of curcumin on injury in hippocampal neurons and expression of blood corticosterone and SGK1 in hippocampal during global cerebral ischemia-reperfusion injury in hypertensive rats

doi:10.12092/j.issn.1009-2501.2019.07.003

Abstract

Abstract:

AIM: To investigate the effects of curcumin on injury in hippocampal neurons and expression of blood corticosterone and serum-and glucocorticoid-inducible kinases1（SGK1） in hippocampal during global cerebral ischemia-reperfusion injury in hypertensive rats. METHODS: Male Wistar-Kyoto rats and male spontaneously hypertensive rats were randomLy divided into five groups (n=30): sham group (W-Sham and S-Sham), ischemia-reperfusion group (W-IR and S-IR), curcumin group (S-Cur). Rats were sacrificed at 3 h, 12 h, 1 d, 3 d, 7 d after reperfusion. Global brain ischemic model was established by 4-VO method. HE Staining was used to observe the vertebral cell morphology in hippocampal CA1 region. Nissl Stainning was applied to detect the average density of vertebral cell in hippocampal CA1 region. The corticosterone in blood and SGK1 in hippocampal were determined by ELISA. At 7d after reperfusion the behavior of the rats was observed. RESULTS:Compared with sham group , the ability to learn and remember was greatly decreased in ischemia-reperfusion group, the number of injured neurons was significantly enhanced , the corticosterone in blood was extremely enhanced（P<0.05）, the expression of SGK1 in hippocampal was significantly increased（P<0.05）. Compared with W-IR, the ability of S-IR to learn and remember was greatly decreased, the number of injured neurons was extremely enhanced, the corticosterone in blood was significantly increased（P<0.05）, the expression of SGK1 in hippocampal was significantly decreased（P<0.05）. The number of injured neurons was greatly declined in S-Cur, the ability to learn and remember was improved , the corticosterone in blood was extremely decreased（P<0.05）, the expression of SGK1 in hippocampal was significantly increased（P<0.05）. CONCLUSION: Inhibition of corticosterone in blood and overexpression of SGK1 in hippocampus may be involved in the mechanism by which curcumin reduces global cerebral IR injuty in hypertensive rats.

Key words: curcumin, cerebral ischemia/reperfusion, cognitive function, corticosterone, serum-and glucocorticoid-inducible kinases1

CLC Number:

R965.2

YU Chenchen, LIU Shuqun, LIU Jianlong, CAO Hong, LIU Xuejiao, CAI Xixi. Effects of curcumin on injury in hippocampal neurons and expression of blood corticosterone and SGK1 in hippocampal during global cerebral ischemia-reperfusion injury in hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(7): 737-743.

References

［1］余结根, 李荣, 梁伟, 等. 醒脑静治疗高血压脑出血的Meta分析［J］.中国临床药理学与治疗学, 2016, 21(4): 417-424.
［2］Grell AS, Mostajeran M, Edvinsson L, et al. Contractile responses in spontaneously hypertensive rats after transient middle cerebral artery occlusion［J］. Pharmacology, 2018, 101(3/4): 120-132.
［3］Ganzer CA，Barnes A，Uphold C, et al.Transient ischemic attack and cognitive impairment: a review［J］. J Neurosci Nurs，2016, 48(6): 322-327.
［4］Sundar Dhilip Kumar S, Houreld NN, Abrahamse H, et al. Therapeutic potential and recent advances of curcumin in the treatment of aging-associated diseases［J］.Molecules, 2018, 23(4): 835-837.
［5］叶克平, 陈春茹, 郑晋伟, 等. 姜黄素对自发性高血压大鼠海马缺血/再灌注损伤神经元凋亡核通路的影响［J］. 中国应用生理学杂志, 2010, 26(4): 416-423.
［6］Pulsinelli WA, Brierley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat［J］. Stroke, 1979, 10(3): 267-272.
［7］Tayebati SK, Tomassoni D, Amenta F.Spontaneously hypertensive rat as a model of vascular brain disorder: microanatomy, neurochemistry and behavior［J］. J Neurol Sci, 2012, 322(1/2): 241-249.
［8］汪小丹, 沈露露, 俞陈陈, 等.姜黄对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响［J］.中华麻醉学杂志, 2014, 34(6): 753-757.
［9］Franco AJ, Chen C, Scullen T, et al.Sensitization of the hypothalamic-pituitary-adrenal axis in a male rat chronic stress model［J］. Endocrinology, 2016, 157(6): 2346-2355.
［10］Semyachkina-Glushkovskaya O, Shirokov A, Gekalyuk A, et al.Stress plays provoking role in hypertension-related stroke: injuries of blood-brain barrier function［C］.Asia Communications and Photonics Conference, 2016, as1k3.
［11］Barra de la Tremblaye P, Plamondon H.Alterations in the corticotropin-releasing hormone (CRH) neurocircuitry: Insights into post stroke functional impairments［J］. Front Neuroendocrinol,2016, 42(10): 53-75.
［12］Joёls M.Functional actions of cortiecosteroids in the hippocampus［J］. Eur J Pharmacol, 2008, 583(2/3): 312-321.
［13］朱洁瑾, 吴湧, 叶晓莉, 等.漆黄素对脑缺血再灌注诱导学习记忆损伤及HPA轴的影响［J］.中国临床药理学与治疗学, 2017, 22(9):1002-1007.
［14］Talarico C, Dattilo V, D'Antona L, et al. SGK1, the new player in the game of resistance: chemo-radio molecular target and strategy for inhibition［J］. Cell Physiol Biochem, 2016, 39(5): 1863-1876.
［15］Anacker C, Cattaneo A, Musaelyan K, et al. Role for the kinase SGK1 in stress, depression, and glucocorticoid effects on hippocampal neurogenesis［J］. Proc Natl Acad Sci USA, 2013,110(21): 8708-8713.
［16］Hinds LR, Chun LE, Woodruff ER, et al.Dynamic glucocorticoid-dependent regulation of Sgk1 expression in oligodendrocytes of adult male rat brain by acute stress and time of day［J］. PLoS One, 2017, 12(4): e0175075.
［17］Xie Y, Jiang D, Xiao J, et al.Ischemic preconditioning attenuates ischemia/reperfusion-induced kidney injury by activating autophagy via the SGK1 signaling pathway［J］. Cell Death Dis, 2018, 9(3): 338.
［18］Zhang W, Qian Cy, Li SQ. Protective effect of SGK1 in rat hippocampal neurons subjected to ischemia reperfusion［J］. Cell Physiol Biochem, 2014, 34(2): 299-312.
［19］俞陈陈, 胡涵, 汪小丹, 等.姜黄素对自发性高血压大鼠脑缺血/再灌注后海马神经元损伤及RANTES表达的影响［J］. 中国应用生理学杂志, 2014, 30(4): 340-344.
［20］姬斌, 刘绪华, 杨芳骅, 等.姜黄素通过抗应激作用改善脑缺血再灌注大鼠工作记忆［J］.中国临床药理学与治疗学, 2014, 19(12): 1321-1325.

[1]	LI Yongliang, LIN Haiyan, ZHANG Lingling, DONG Yilong. Effects of preoperative dezocine patient-controlled analgesia pump on stress response and cognitive function in elderly patients with lower extremity fractures [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(3): 302-306.
[2]	SHEN Longde, LI Ailu, LI Lichun, WU Yinyin, SHEN Longyuan, JIANG Qiuxia, HE Jinxi, WU Zhifen, YU Liji, ZHANG Xiaohong. Experimental study on the effect of curcumin on endometriosis in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(8): 862-868.
[3]	ZHANG Rui. Effects of dexmedetomidine on cognitive dysfunction and attentional network function in patients with ischemic cerebrovascular disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(7): 815-820.
[4]	HU Defeng, WANG Yihu, LONG Xiaoyu, WANG Guangshui. Intervention effect of modified Ganmaidazao Tang combined with cognitive behavioral intervention on ultra-high risk for developing psychosis and the effects on cognitive function, quality of life and serum levels of 5-HT, MT and TSH [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(5): 546-553.
[5]	QIU Fei, GAO Dan, YANG Dongmei, ZHONG Xiaomei, TANG Sha, YIN Huiming, ZHANG Zaiqi. Effects of curcumin on proliferation of VSMCs and expression of PTEN induced by PDGF-BB [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1358-1363.
[6]	LIU Xiaoyan, TIAN Ye, LIU Jifei, SU Gang, ZHANG Zhenchang. Research progress on the protective mechanisms of curcumin on cerebral ischemia-reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1305-1309.
[7]	. Effects and mechanisms of nicotinic acid curcumin on apoptosis induced by low shear stress in human umbilical vein endothelial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 855-861.
[8]	CHEN Juanyan, SONG Shuijiang, XU Dongjuan, XU Qiong, LI Hongfei. Effects of fasudil combined with rosuvastatin in the treatment of vascular dementia on patients' cognitive function, C-reactive protein and oxidative stress [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(10): 1153-1158.
[9]	MO Yali, XIA Yong, LIU Yi, TAO Yunhai, JIN Man. Effects of huperzine A combined with escitalopram on clinical efficacy and cognitive function in depression patients [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(9): 1040-1044.
[10]	LI Xujiong, LIU An, CHEN Yunxia, LI Shufen. Curcumin alleviate intrapulmonary inflammation in rat experimental hepatopulmonary syndrome [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 738-742.
[11]	LI Xu, Dai Yi, HUANG Jiafeng, WEN Chunjie, ZOU Zhen, Wu Lanxiang, WANG Guo, ZHOU Honghao. Curcumin increases MDR1 expression in breast cancer MCF-7 and MCF-7/DOX cells through regulating histone acetylation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 512-517.
[12]	CHEN Yongcai, ZHOU Bin, CHEN Xuemin, WENG Jinyue. Effect of curcumin on the human colon cancer cells SW480 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 526-530.
[13]	DENG Yingfeng. Application of dexmedetomidine in elderly patients during ESD operation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 580-583.
[14]	XU Buyuan, PAN Xiange, CHEN Chongbie, CAI Min, XU Lu. Effects of curcumin on VEGF and endostatin expression of Lewis lung carcinoma [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(2): 151-154.
[15]	YOU Kuangzhang, CHEN Bin, BAO Qianqian. Effect of sevoflurane combined with propofol on postoperative cognitive function and serum oxidative stress level in elderly patients with non small cell lung cancer after thoracic surgery [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(2): 190-193.