Abstract: Aim To explore the function of immune modulation of anti-HBV iRNA in patient s with chronic hepatitis B(CHB).Methods Peripheral blood lymphocyte iRNA was prepared from anti-HBs positive human body with HBV complete clearance after HBV infection(h-iRNA). The effect of h-iRNA on HBV specific lymphocyte proliferative response of peripheral lymphocyte from patient s with CHB was observed by using MT T method and was compared with that by HBV specific iRNA from animal immunized only by HBsAg (a-iRNA).Results Both h-iRNA and a-iRNA increased the level of peripheral lymphocyte proliferative response to HBsAg in patient s with CHB to some degree.In grouPof HBcAg,only h-iRNA showed its enhancement of HBcAg specific lymphocyte proliferative response.Conclusion sh-iRNA can increase HBV specific lymphocyte proliferative response in patients with CHB and the function of increasing HBcAg specific lymphocyte proliterative response contributes to HBV clearance.

Key words: hepatitis B, HBV protective antibody, iRNA, lymphocyte proliferative response