Effects of chloride channels blockers on Ca2+ influx induced by adrenaline in A10 cells1

Abstract

Abstract: AIM: To investigate the relationship be-tween chloride channels and the Ca²⁺ influx induced by a-drendine(Adr).METHODS: The effects of drugs on Adr-induced Ca²⁺ influx were investigated with Fura-2 fluorescence technique.RESULTS: Adr-induced Ca²⁺ influx was inhibited by nifedipine, SK&F96365, NFA and furosemide in a concentration manner respectively; Ca²⁺ influx could be further inhibited by NFA or furosemide after the maximal inhibition by SK&F96365;SK&F96365 also could further inhibit the Ca²⁺ influx which had been inhibited by NFA or furosemide.Genistein and vanadate could reduce or increase the Ca²⁺ influx respectively. .CONCLUSION: Ca²⁺ influx induced by Adr is related to VDC and ROC, and chloride channels involves in the processes.The levels of tyrosine phosphoralation affect the Ca²⁺ influx.

Key words: vascular, Cl^- channels, cell, Ca²⁺

CLC Number:

R332
Q463

RUAN Hong-Mei, GUAN Yong-Yuan, HE Hua, QIU Qing-Ying. Effects of chloride channels blockers on Ca²⁺ influx induced by adrenaline in A10 cells¹[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(2): 106-109.

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Effects of chloride channels blockers on Ca²⁺ influx induced by adrenaline in A10 cells¹

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