Abstract: AIM: To study the modulation on mRNA expression of muscarinic acetylcholine receptors in bovine aorta endothelial cells by carbachol and pilocarpine.METHODS: Total RNA was extracted from the bovine aorta endothelial cells having been incubated with carba-chol and pilocarpine at the dose of 10^-4 mol·L^-1for 10hr. The expression of muscarinic acetylcholine receptor subtypes mRNA was measured by RT-PCR.RESULTS: The mRNA expression of five muscarinic acetylcholine receptors were allmeasuireed. These five genes were up-reg ulated after incubated with carbachol and pilocarpine atthe dose of 10^-4 mol·L^-1 for 10hr and therre was no diference in them.CONCLUSION: Carbachol and pilocarpine has the same effect on genes expression of muscarinic acetylcholine receptor subtypes in aorta endothelialcells. Their effects on muscarinic acetylcholine receptorsubtypes can not explain the influence to vascular tension

Key words: muscarinic acetylcholine receptor, endothelial cells, carbachol, pilocarpine, gene expression