Abstract: AIM: To screen anti-inflammatory Chi-nese traditional herbs targeted CpG ODN using biosensortechnology.METHODS: Firstly,CpG ODN was immo-bilized on surface of biotin cuvette.Aqueous extractions of seventy-eight traditional Chinese herbs were tested us-ing affinity biosensortechnology.Then aqueous extrac-tions of the herbswith high affinity for CpG ODN were in-cubated with CpG ODN(33μg·ml^-1)at 37 ℃ for30 min,subsequently the mixtures were tested using affinity biosensortechnology.RESULTS: Among the seventy-eight Chinese traditional herbs,fourteen herbs such as ra-dix paeoniae rubra,rhubarb,and terminaliae immaturus possessed the higherbinding activity to CpG ODN,and nine herbs such as Java Brucea fruit,radix paeoniae rubra,and radix sanguisorbae possessed largeramount of effective binding-CpG ODN components.CONCLUSION: Nines kinds of Chinese traditional medicines pos-sess the highly content components with highly specifically affinities to CpG ODN and it provides experimental base forfurtherseparation anti-inflammatory component from Chinese traditional medicine.

Key words: biosensor, Chinese traditional medi-cine, CpG ODN, affinity, sepsis, screening