Effects of 5-Fluorouracil on cell proliferation and potassium channel function in oral cancer cell line Tb

Abstract

Abstract: AIM: To evaluate the effects of 5-Fluorouracil (5-FU) on cell proliferation and potassium channel function in oral cancer cell line Tb.METHODS: After treatment with 5-FU for 24 h, the proliferation of Tb cells was determined by MTT and clone formation assay, the membrane potential and potassium channel current of Tb cells were recorded by whole-cell patch clamp technique.RESULTS: Compared with the control group (CON), 5-FU inhibited proliferation of the Tb cells.At the same time the membrane was depolarized and the potassium channel current significantly decreased compared with the CON group.CONCLUSION: Potassium channel may play an important role in 5-FU-inhibited the proliferation of Tb cells.

Key words: 5-Fluorouracil (5-FU), Tb cells, proliferation, potassium channel, patch clamp technique

CLC Number:

R979.14

MA Yu-guang, WU Li, WANG Yu-cheng, MA Da-chang, WU Li, WANG Li-jing. Effects of 5-Fluorouracil on cell proliferation and potassium channel function in oral cancer cell line Tb[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(8): 890-893.

References

1 Wonderlin WF, Strobl JS.Potassium channels, proliferation and G1 progression[J].J Membr Biol, 1996;154:91-107
2 Bordey A, Sontheimer H.Electrophysiological properties of human astrocytic tumor cells in situ:enigma of spiking glial cells [J].J Neurophysiol, 1998;79:2782-93
3 Yao X, Kwan HY.Activity of voltage-gated K+channels is associated with cell proliferation and Ca²⁺influx in carcinoma cells of colon cancer[J].Life Sci, 1999;65:55-62
4 Klimatcheva E, Wonderlin WF.An ATP-sensitive K+current that regulates progression through early G1 phase of the cell cycle in MCF-7 human breast cancer cells[J].J Membr Biol, 1999;171:35-46
5 吴军正, 陈建元, 李峰, 李焰, 张洪.舌癌脑转移细胞系Tb的建立及形态和生长特性[J].实用口腔医学杂志, 1999; 15:452-3
6 Fu ZJ, Xie MJ, Zhang LF, Cheng HW,Ma J.Differential activation of potassium channels in cerebral and hindquarter arteries of rats during simulated microgravity[J].Am J Physiol Heart Circ Physiol, 2004;287:H1505-15
7 Pancrazio JJ, Viglione MP, Tabbara IA, Kim YI.Voltage-dependent ion channels in small-cell lung cancer cells[J].Cancer Res, 1989;49:5901-6
8 Xu R, Roh SG, Loneragan K, Pullar M, Chen C.Human GHRH reduces voltage-gated K+currents via a non-cAMP-dependent but PKC-mediated pathway in human GH adenoma cells [J].J Physiol, 1999;520:697-707
9 Meyer R, Heinemann SH.Characterization of an eag-like potassium channel in human neuroblastoma cells [J].J Physiol, 1998;508:49-56
10 Kohler R, Degenhardt C, Kuhn M, Runkel N, PaulM, Hoyer J.Expression and function of endothelial Ca²⁺-activated K+channels in human mesenteric artery:a single-cell reverse transcriptase-polymerase chain reaction and electrophysiological study in situ[J].Circ Res, 2000;87:496-503
11 Skryma R, Van Coppenolle F, Dufy-Barbe L, Dufy B, Prevarskaya N.Characterization of Ca²⁺-inhibited potassium channels in the LNCaP human prostate cancer cell line[J].Receptors Channels, 1999;6:241-53
12 韩英, 郎兵, 吴汉平, 周炜, 高瞻, 周士胜, 等.人胃癌SGC7901 细胞膜钾离子通道的特性[J].第四军医大学学报, 2002;23:608-10
13 Jensen BS, Odum N, Jorgensen NK, Christophersen P, Olesen SP.Inhibition of T cell proliferation by selective block of Ca⁽²⁺⁾-activated K⁽⁺⁾ channels[J].Proc Natl Acad Sci USA, 1999;96:10917-21

[1]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[2]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[3]	DING Jiamin, WANG Youdi, ZHAO Xiali, JIANG Genfeng, CHEN Siwen, HONG Chengcheng, LI Shuqin, HU Weihua. Study on the treatment of thin endometrium with growth hormone and vitamin E [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 857-862.
[4]	WANG Xinli, XU Xiaqing, FANG Hanbing, GUO Yuzhong. Study of TPA on enhancing the anti-tumor effects of cisplatin and reducing its renal toxicity [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(5): 535-543.
[5]	LI Yanyan, FANG Xiaojie, YIN Xin, SUN Xi, RAO Chunhui. circZNF124 regulates the proliferation, migration and invasion of colorectal cancer SW620 cells by targeting miR-4262 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1231-1239.
[6]	LEI Jing, HE Ping, CAI Wenlin, ZHANG Yongzhe, ZHANG Beiru, LIU Dajun. Cordyceps sinensis extracts attenuates HBx-induced mesangial cell proliferation and extracellular matrix synthesis by suppression of the PI3K/Akt pathways [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1247-1254.
[7]	CHEN Linjun, CHEN Wenbin. Jolkinolide B inhibits the proliferation and metastasis of colon cancer cells via blocking PI3K/Akt/NF-κB pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(3): 250-257.
[8]	PENG Fusheng, HUANG Xiaohui, LI Peng, TANG Jian'er. miR-34a inhibits proliferation of prostate cancer LNCaP cells by regulating androgen receptor gene [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(1): 10-17.
[9]	WANG Xianghai, XING Min, LYU Tangfeng, LIU Hongbing, SONG Yong. Effects of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(8): 841-849.
[10]	HOU Bin, HUANG Weiping, YIN Zhongpu, HU Shousen. lncRNA PCAT19 inhibits the proliferation and invasion of nasopharyngeal carcinoma cells by adsorbing miR-142-5p and regulating the expression of ING3 gene [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(7): 721-727.
[11]	OU Jun, HOU Wenwen, TANG Jingwen, LI Jiaping, XU Qingyang. miR-106a regulates the proliferation of ovarian granulosa cells by targeting TIMP-2 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(5): 527-532.
[12]	HUANG Kang, LI Ling, YE Qifa, PENG Guizhu. Expression of long non-coding RNA LINC00844 in hepatocellular carcinoma and its inhibiting effect on cell proliferation and migration [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(4): 366-372.
[13]	SONG Hui, WANG Yinghao. Resveratrol inhibits the proliferation of retinoblastoma cells via miR-937/FOXQ1 pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(2): 174-181.
[14]	CHENG Jianping, LI Zhen, ZHAO Xiaolin, YANG Mengyuan, JI Xiwei, YU Jiufei. Research on the expression of miR-186-5p in serum of patients with colon cancer#br# [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(10): 1097-1104.
[15]	ZHANG Kang, HE Binjun, HU Wenbin, HAN Xiaoliang. Silencing carbonic anhydrase 1 inhibits the proliferation, invasion and migration of A549 cells in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(1): 55-60.