Effects of tubeimoside on adhension, invasion and migration of a human highly metastatic giant lung carcinoma cell line PGCL3

Abstract

Abstract: AIM:To design to investigate the effects of tubeimoside on adhesion, invasion and migration of PGCL3 cells .METHODS :The cells grow inhibition me- diated by tubeimoside was measured by MTT assay after treatment with tubeimoside .The effects of tubeimoside on adhesion, invasion and migration of PGCL3 cells were de- termined using analysis of adhesion-related basement membrane components fibronectin and laminin, reconsti- tuted basement membrane invasion assay, and Transwell model, respectively .RESULTS:Tubeimoside displayed growth inhibitory activity against PGCL3 cells with IC₅₀ values of 16 .77, 14 .62, and 14 .46 μmol·L^-1 for 24, 48, 72 hours of tubeimoside treatment .1 .25, 2 .50 and 5 .00 μmol·L^-1 of tubeimoside inhibited PGCL3 cells to invade the reconstituted basement membrane by 20 .6 %, 30 .6 %, 46 .8 %, and decreased the percentages of loco- motion and migration of PGCL3 cells by 14 .2 %, 24 .7 %, 42 .5 %, respectively .In addition, tubeimoside inhibited PGCL3 cells to adhere to the basement mem- brane components fibronectin and laminin, and inhibited the activity of type IV collagenase and its secretion by PGCL3 cells in a dose-dependant manner.CONCLU- SION:Tubeimoside can significantly inhibit the adhe- sion, invasion and migration of PGCL3 cells, and the sup- pression of adhesion of PGCL3 cells to fibronectin and laminin and of secretion and activity of type IV collage- nase in PGCL3 cells mediated by tubeimoside may be re- sponsible for the inhibition of adhesion and invasion of PGCL3 cells .

Key words: tubeimoside , adhesion , invasion , mi- gration , type IV collagenase, PGCL3 cells

CLC Number:

R979 .1

WANG Chang-xiu, MA Run-di, YU Li-jian. Effects of tubeimoside on adhension, invasion and migration of a human highly metastatic giant lung carcinoma cell line PGCL3[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(1): 39-44.

References

1 赵学敏.本草纲目拾遗[ M] .北京:人民卫生出版社, 1983: 123-7
2 王永清, 于立坚, 朱娟莉, 杨世勇.土贝母皂苷提取物的抗肿瘤作用[ J] .陕西新医药, 1981 ; 10: 55-6
3 Kong F, Zhu D, Xu R, Fu Z, Zhou L, Iwashita T, et al . Structural study of tubeimoside 1, a constituent of tu-be i - mu [ J] . Tetrahedron Lett, 1986 ; 27: 5765-9
4 Kaisai R, Miyakoshi M, Nie RL, Zhou J, Matsumoto K, Morita T, et al . Saponins from bolbostemma:cyclic bisdesmosides, tube- imosides Ⅱ and Ⅲ[ J] . Phytochemistry, 1998; 27 : 1439 -46
5 马润娣, 于立坚, 王永清, 西野辅翼, 高安淳子.土贝母皂苷甲素对鼠耳炎性水肿和小鼠皮肤促癌过程的强抑制作用[ J] .科学通报, 1991 ; 36: 1421-4
6 Yu LJ, Ma RD, Wang YQ. Potent anti-tumor activity and low toxicity of tubeimoside 1 isolated from Bolbostemma paniculatum [ J] . Planta Med, 1994 ; 60: 204 -8
7 Yu LJ,Ma RD, Yu TX. Induction of morphological and functional differentiation of human promyelocytic leukemia cells( HL-60) by tubeimoside I[ J] . Planta Med, 1996 ; 62: 119-21
8 胡章, 马润娣, 于立坚.土贝母苷甲对人 HL-60 髓性白血病细胞周期与凋亡的影响[ J] .中国肿瘤临床, 2003; 30: 163-6
9 Ma RD, Weng XY, Yu LJ, Su WM, Shao HY, Liao MN, et al . 中国临床药理学与治疗学 2006 Jan; 11( 1) · 43 ·Apoptosis of human nasopharyngeal carcinoma CNE-2Z cell line induced by tubeimoside Ⅰ isolated from Bolbostemma panicula- tum[ J] . US ChinJ Lymphol Oncology, 2003; 2 : 11-8
10 Ma RD, Yu LJ, Su WM, Shao HY, Liao MN, He DM, et al . Induction of cell cy cle arrest and apoptosis by tubeimosideⅠ iso- lated from Bolbostemma paniculatum in heLa cells[ J] .Chin J Clin Pharmacol Ther, 2004 ; 9: 261-9
11 宋刚, 马润娣, 于立坚.土贝母皂苷对人低分化上皮样鼻咽癌细胞( CNE-2Z) 微管的作用[ J] .中国临床药理学与治疗学, 2005; 10: 617 -21
12 Wang F,Ma RD, Yu LJ.Role of mitochondria and mitochondri- al cytochrome c in tubeimoside i-mediated apoptosis of human cervical carcinoma hela cell line[ J] . Cancer Chemother Pharma- col, 2005, in press
13 Yu TX, Ma RD, Yu LJ .Structure-activity relationship of tube- imosides in anti-inflammatory, antitumor, and antitumor-promot- ing effects[ J] . Acta Pharmacol Sin, 2001; 22 : 463-8
14 吴秉铨, 孙毓恺, 郑杰, 王洁良, 方伟岗, 唐尧, 等.裸鼠体内建立的人类高转移癌系[ J] .中华肿瘤杂志, 1985 ; 7: 324 -32
15 Hansen MB, Nielsen SE, Berg K.Re-examination and further development of a precise and rapid dye method for measuring cell growth cell kill[ J] . J Immunol Methods, 1989 ; 119: 203-10
16 Albini A, Iwamoto Y, Kleinman HK, Martin GR, Aaronson SA, Kozlowski JM, et al . A rapid in vitro assay for quantitating the invasive potential of tumor cell[ J] .Cancer Res, 1987 ; 47 : 3239 -45
17 Stracke ML, Soroush M, Liotta LA, Schiffmann E.Cytoskeletal agents inhibit motility and adherence of human tumor cells[ J] . Kidney Int, 1993; 43: 151 -7
18 Davies B, Miles DW, Happerfield LC, Naylor MS, Bobrow LG, Rubens RD, et al . Activity of type IV collagenases in benign and malignant breast disease[ J] . Br J cancer, 1993; 67 : 1126-31
19 周龙恩, 刘红岩, 徐少锋, 付招娣, Kurt S Zaenker, 韩锐. 紫杉醇对 MDA-MB-435 人乳腺癌高转移细胞粘附、侵袭及迁移能力的影响[ J] .中国药理学与毒理学杂志, 2001; 15: 51-5
20 杨萍, 于廷曦, 马润娣, 于立坚, 苏伟明, 邵海艳, 等.土贝母皂苷诱导人宫颈癌 HeLa 细胞周期阻滞和细胞凋亡 [ J] .癌症, 2002 ; 21: 346-50
21 Hendrix MJ, Wood WR, Seftor EA, Lotan D, Nakajima M, Misi- orowski RL, et al .Retinoic acid inhibition of human melanoma cell invasion through a reconstituted basement membrane and its relation to decreases in the expression of proteolytic enzymes and motility factor receptor[ J] . Cancer Res, 1990; 50: 4121-30
22 Reich R, Thompson EW, Iwamoto Y, Martin GR, Deason JR, Fuller GC, et al .Effects of inhibitors of plasminogen activator, serine proteinases, and collagenase IV on the invasion of base- ment membranes by metastatic cells[ J] .Cancer Res, 1988; 15; 3307-12
23 Parsons SL, Watson SA, Brown PD, Collins HM, Steele RJ.Ma- trix metalloproteinase[ J] . Br J Surg , 1997; 84: 160 -6

[1]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[2]	SUI Yuxia, ZHUANG Jie, ZHUANG Min, HUANG Gui. Delicaflavone inhibits the invasion and migration of gefitinib-resistant lung cancer PC-9/GR cells by regulating epithelial-mesenchymal transition via PI3K/Akt/mTOR pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 614-621.
[3]	SHI Jingbin, XIONG Yang. Research progress on interaction between tumor microenvironment and breast cancer cells leading to tumor metastasis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(5): 562-574.
[4]	YE Xiaodi, MIAO Yunping, CHEN Aiying, CHENG Min, TIAN Xuejun, ZHENG Gaoli . Optimization and verification of orthotopic non-muscle invasive bladder cancer model in nude mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(4): 473-480.
[5]	LI Yanyan, FANG Xiaojie, YIN Xin, SUN Xi, RAO Chunhui. circZNF124 regulates the proliferation, migration and invasion of colorectal cancer SW620 cells by targeting miR-4262 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1231-1239.
[6]	HOU Bin, HUANG Weiping, YIN Zhongpu, HU Shousen. lncRNA PCAT19 inhibits the proliferation and invasion of nasopharyngeal carcinoma cells by adsorbing miR-142-5p and regulating the expression of ING3 gene [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(7): 721-727.
[7]	SHEN Cheng, YANG Nianzhao, WANG Minghai, WU Lisheng, ZHU Zhiqiang. Inhibiting RNA methylase NSUN2 reduce the invasion and migration of gastric cancer cell [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(4): 380-386.
[8]	ZHANG Kang, HE Binjun, HU Wenbin, HAN Xiaoliang. Silencing carbonic anhydrase 1 inhibits the proliferation, invasion and migration of A549 cells in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(1): 55-60.
[9]	WANG Qingwei, DING Jin, LV Yuanyuan, LUO Xianchen, NI Guantai. Effects of PHA-767491 on the inhibition of CDC-7 expression in endometrial cancer ishikawa cell line [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(8): 866-871.
[10]	ZHOU Qingying, XU Naifen, ZHENG Dingqin, PAN Qizhuang, ZHANG Zhigang, WANG Shuo. Fluorouracil regulates VHL/HIF signaling pathway and inhibits proliferation, invasion and metastasis of renal carcinoma cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(8): 872-879.
[11]	Yuan ShaoFei. miR-1304 inhibits non-small cell lung cancer cell invasion and proliferation in vitro via targeting heme oxygenase-1 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 383-390.
[12]	CHEN Yuequn, ZHAI Hongbo, TONG Jinyi, QU Wanglei. Raddeanin inhibits the invasion and metastasis of HEC-1-B cells in human endometrial carcinoma by inhibiting the JAK/STAT3 signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 391-396.
[13]	LI Peng, YANG Ronghua, ZHANG Minghua, XIAO Xin, TANG Jianer. Expression of miR-34a-5p in bladder cancer and its effect on invasion and migration of bladder cancer cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 403-410.
[14]	QIAN Wanfeng, WANG Xiufeng, CHEN Xuepeng. Expression of miR-19b in osteosarcoma and its effect on invasion and migration of osteosarcoma Saos-2 cells by regulating KLF13 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1242-1248.
[15]	ZHAO Qiaosu，YE Hua，SUN Qinxue. Effects of circadian clock gene Period2 on Warburg effect and metastasis in hepatocellular carcinoma cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(10): 1134-1141.