Abstract: AIM: To study the effects of resveratrol trinocotinate(ResT), a structure modification compound on the basis of resveratrol, on proliferation inhibition and apoptosis in HepG2 cell line.METHODS: HepG2 cell were cultivated with different concentration of ResT at the different time in vitro.The proliferation inhibition was measured by MTT assay.Hochest staining method was used to determine the apoptosis, and then FCM was used to analyzed the cell cycle distribution and apoptosis rate, Colorimetric assay wasemployed to detect the activation of Caspase-3.RESULTS: ResT caused growth inhibition in a time and dose-dependent manner.After treatment with ResT for 24 h, some cells appeared apoptotic characteris-tics.The flow cytometric profiles revealed that ResT in-duced cell into G₁ -phase and cells in S-phase were de-creased, Apoptotic peaks were observed in the cell cycle analysis.The apoptosis rates were 8.7%, 21.1% and 32.7% respectively after treatment for 12, 24, 48 h. Caspase-3was also activated.CONCLUSION: Induction of apoptosis and G₁ -phase cell cycle arrest may be respon-sible for the growth inhibition effect of ResT in HepG2 cell.

Key words: resveratrol, resveratrol trinocotinate, HepG2, apoptosis, caspase-3