Welcome to Chinese Journal of Clinical Pharmacology and Therapeutics,Today is Chinese

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2006, Vol. 11 ›› Issue (8): 940-942.

Previous Articles     Next Articles

Inhibitory effect of licoric extracts on activity of tyrosinase

SHEN Jun, HE Li-ping, ZHANG Rui-bin   

  1. Department of Sanitary Chemistry , School of Public Health, Tianjin Medical University , Tianjin 300070 , China
  • Received:2006-05-18 Revised:2006-06-16 Online:2006-08-26 Published:2020-11-05

Abstract: AIM: To study the inhibitory effects of the licoric extracts on the activity of tyrosinase, which is constituted of monophenolase and diphenolase.METHODS: The 50%(V/V) alcohol is used as extractant.To investigate the effects of the licoric extracts on the monophenolase and diphenolase activity, the absorbance of the solution containing several concentrations of the licoric extracts was measured at 475 nm and 390 nm, using the Ltyrosine and pyrocatechin as the substrate, respectively.RESULTS: The licoric extracts has showed a strongly inhibition on the activity of tyrosinase, either monophenolase or diphenolase.The IC50 is 1.94 and 2.94mg·ml-1 , respectively.CONCLUSION: The licoric extracts has inhibitory effects on both monophenolase and diphenolase.The diphenolase activity test system using pyrocatechin as the substrate is a useful procedure to study the activity of tyrosinase.

Key words: tyrosinase, licorice root, VitC, melanin

CLC Number: