Agonists of group II and III metabotropic glutamate receptors reverse LPS-induced glutamate uptake inhibition in C6 glioma cells

Abstract

Abstract: AIM:To study whether agonists of group II and III metabotropic glutamate receptors (mGluRs) exert effects on LPS-induced glutamate uptake inhibition in C6 glioma cells.METHODS:The glutamate uptake into C6 glioma cells was measured by uptake of [³H]-D, Lglutamate; and the apoptosis and the viability of C6 glioma cells were investigated by Hoechst33342 and MTT methods, respectively.RESULTS:LPS (4, 6 μg/mL) inhibited glutamate uptake significantly compared with that in the control group without effect on the apoptosis and viability of C6 glioma cells. Pretreatment of C6 glioma cells with group II and III mGluRs agonists DCG-IV (100 μmol/L) and L-AP4(100 μmol/L) reversed LPS-induced glutamate uptake inhibition. These recovery effects were abolished by their respective antagonists APICA and MSOP.CONCLUSION:Activation of group II and III mGluRs recovers LPS-induced glutamate uptake inhibition in C6 glioma cells, suggesting the enhancement of glutamate uptake is involved in neuroprotective roles exerted by group II and III mGluRs agonists.

Key words: metabotropic glutamate receptors, lipopolysaccharide, C6 glioma cells, glutamate uptake

CLC Number:

R965. 2

ZOU Jing, YAO Hong-Hong, HU Gang. Agonists of group II and III metabotropic glutamate receptors reverse LPS-induced glutamate uptake inhibition in C6 glioma cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(6): 626-629.

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