Abstract: AIM:To evaluate the hypoglycemic mechanism of S-8300 in alloxan diabetic mice. METHODS:The effects of S-8300 on diabetic mice were investigated by observing the change of cholesterol, triglyceride, free fatty acid, superoxide dismutase, malondialdehyde, ATPase activity and the hemolysis of red blood cells. RESULTS: The levels of cholesterol, triglycerides, free fatty acid in plasma and malondialdehyde in tissues were significantly decreased and the activities of superoxide dismutase and ATPase in the liver and kidney were significantly increased in diabetic mice treated with S-8300. S-8300 exhibited a significant potency in inhibiting autoxidation haemolysis and the red cellular membrane injury by H₂O₂ in vitro. CONCLUSION:The hypoglycemic mechanism of S-8300 is relate to its decreasing plasma lipid of alloxandiabetic mice and alleviating the harm of free radicals.

Key words: S-8300, mechanism, diabetes, lipid peroxidation