Purification of antineoplasmic activity protein from the venom of Wannan Agkistrodon and the study in vitro

Abstract

Abstract: AIM: To purify the antineoplasmic active protein from the venom ofWannan Agkistrodon and to study the activity in vitro.METHODS: An antineoplasmic activity proteinum was isolatded from the venom of Wannan Agkistrodon by DEAE-sepharose Fast Flow and SP-sepharose Fast Flow column chromatography and Sephadex G-75 gel filtration chromatography, the generation depressant effects of this proteinum were detected by CCK-8 method in some tumor cells such as K562, SW480, SGC7901, HepG2 were cultured in vitro.RESULTS: An antineoplasmic activity ingredient named ATF1-c was discovered, its molecular weight was 23700.The generation depressant effects of K562, SW480, SGC7901, HepG2 cells cultured in vitro were detected by CCK-8 method, and there were dosage-time dependent relationships.CONCLUSION: ATF1-c has significant inhibited and lethal effect to human cancer cells cultured in vitro.

Key words: Agkistrodon, Separationandpurification, CCK-8colorimetry, K562, SW480, SGC7901, HepG2

CLC Number:

R965.2

PAN Xue-bing, CHEN Dong-yun, HUAI Jian-guo, JI Zhao-ning. Purification of antineoplasmic activity protein from the venom of Wannan Agkistrodon and the study in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(9): 991-995.

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