Anticancer activity of extractive purified from Agkistrodon acutus Venom in vitro

Abstract

Abstract: AIM: To purify snake venom metalloproteinase x (SVMP-x) from Agkistrodon acutus Venom and investigate its anticancer activity in vitro.METHODS: The SVMP-x was separated from Agkistrodon acutus Venom by ion exchange using DEAE-Sepharose FF and SP-Sepharose FF and further purified by gel filtration through sephadex G75 columns.Different origins of tumor cell lines were cultured with different concentrations of SVMP-x.Vehicle and 5-FU were used as negative and positive control respectively.The in vitro anticancer activity of SVMP-x was determined by CCK-8 assay.RESULTS: SVMP-x was purified from Agkistrodon acutus Venom.CCK-8 assay demonstrated that SVMP-x inhibited the proliferation of SGC7901, SW480, SH-SY5Y and B16 in a dose-dependent manner.The inhibition rates of SVMP-x to SGC7901 and B16 were significantly higher than that of 5-FU positive control.SVMP-x inhibited the proliferation of SGC7901 in a time-and dose-dependent manner.CONCLUSION: An anticancer component SVMP-x is successfully purified from Agkistrodon acutus Venom and SVMP-x inhibits the proliferation of a variety of tumor cell lines in vitro.

Key words: Agkistrodon acutus, SVMP-x, purification, anticancer

CLC Number:

R965.2

CHEN Dong-yun, PAN Xue-bing, JI Zhao-ning. Anticancer activity of extractive purified from Agkistrodon acutus Venom in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 37-41.

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