Study on the hypoglycemic mechanism of the fusion protein HSP65 -6 ×P277 in NOD mice

Abstract

Abstract: AIM: To evaluate the hypoglycemic mechanism of the fusion protein HSP65 -6 ×P277 in NOD mice. METHODS: The fusion protein was used to immunize 4-week old female NOD mice with three i. n.inoculations in the absence of adjuvants.The effects of HSP65 -6 ×P277 on NOD mice were investigated by observing the change of blood glucose, cytokine levels, antibody types and degree of insulitis. RESULTS: The sera collected from HSP65 -6 ×P277 treated mice, contained relatively high levels of interleukin IL-4 but low levels of IL-2, and the antibody types were almost exclusively of the IgGl and IgG2b subclass, but at very low levels of IgG2a.T cells from HSP65 -6 ×P277 treated mice when incubated with HSP-peptide conjugate showed an increase in IL-10 secretion and a decrease in interferon (IFN)-γsecretion. HSP65 -6 ×P277 treatment reduced insulitis and T1DM incidence. CONCLUSION: The hypoglycemic mechanism of the fusion protein HSP65 -6 ×P277 is related to its inducing Th2 responses.

Key words: heat shock protein 65, P277, Th1/Th2 cells, cytokines

CLC Number:

R965

JIN Liang, WANG Yu, ZHU Ai-hua, LIU Jing-jing. Study on the hypoglycemic mechanism of the fusion protein HSP65 -6 ×P277 in NOD mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1142-1150.

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