Abstract: AIM: To investigate the preventive effects of Extract of Ginkgo Biloba (EGB761) on the cell aging of cardiomyocytes induced by D-galactose and its latent mechanism. METHODS: Cell aging of cultured neonatal rat cardiomyocytes was created by 5 g/L D-galactose. EGB761 was added to cultured cardiomyocytes at different concentration (5, 10 and 20 μg/mL, respectively) for 48 hours. Cell aging was determined by a senescent β-galactosidase staining. Advanced glycation end products (AGEs) were quantitative analyzed by ELISA. The morphology and beat of cardiac myocytes were observed under an invert microscope. RESULTS: D-galactose resulted in a significantly increased β-galactosidase positive cardiomyocytes compared with the controls (75.6%±4.9% vs 17.15%±2.9%, P<0.01), concomited with elevated AGEs[(703±32)vs (93±26) pg/mL, P<0.01], abnormal cellular morphology and beat rate. EGB761 treated groups showed significant decrease in both β-galactosidase positive cells(57.7%±7.9%, 49.7%±9.2%, 34.0%±6.6%, P<0.01) and AGEs levels [(404±33), (357±25), (249±77) pg/mL, respectively, all P value were less than 0.01], accompanied by relative normal morphology and beat rate. CONCLUSION: EGB761 has anti-aging actions on cardiomyocytes through inhibit non-enzymatic reactions.

Key words: Extract of Ginkgo Biloba, Advanced glycation end products(AGEs), Cardiomyocyte, Aging, β-galactosidase