Effect of Juglone on the angiogenesis mmicrovessel structure of rat aorta and chick chorioallantoic membrane

Abstract

Abstract: AIM: To investigate the effect of Juglone on the growth and migration of endothelial cell line EAHY.926, the microvessel structure of rat aorta and chick chorioallantoic membrane angiogenesis to provide pre-experimental data for the research of the mechanism of Juglo-ne in preventing tumor angiogenesis factor. METHODS: The effect of a series of concentration of Ju glone on the growth of EAHY.926 were measured by MTT. Serum-free culture of the rat aorta was conducted, with 100 μmol/L,50 μmol/L,25 μmol/L and 12.5 μmol/L dosages of Juglone added, to observe its effect on the microvessel structure. 7-day-old chick embryo were elected for experimental angiogenesis model. RESULTS: We found that 200 μmol/L, 100 μmol/L and 50 μmol/L dosages of Juglone could effectively inhibit the proliferation of EAHY.926 in a dose-dependent manner(P<0.05), with an IC₅₀ of 122.848 μmol/L according to the results of MTT. While 25 μmol/L,12.5 μmol/L and 6.25 μmol/L dosages of Juglone could promote growth of EAHY.926(P<0.05). 100 μmol/L,50 μmol/L and 25 μmol/L dosages of Juglone could completely inhibit the formation of new vessels,and 12.5 μmol/L dosages of Juglone could reduce the number of endothelial cells. 200 μmol/L,100 μmol/L,50 μmol/L and 25 μmol/L Juglone had vascular stimulation, hemolyzation, agglutination as a dose-dependent manner. 12.5 μmol/L Juglone could inhibit angiogenesis. CONCLUSION: Juglone can inhibit the formation of new vessels.

Key words: Juglone, Prevent vessel formation, Aorta, EAHY.926, Chick chorioallantoic membrane

CLC Number:

R965.2

CHEN Li, ZHANG Jian, WANG Si-ying, HUANG De-wu, GU Wei-wang. Effect of Juglone on the angiogenesis mmicrovessel structure of rat aorta and chick chorioallantoic membrane[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(4): 403-409.

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