Protective effects of icariin on cerebral mitochondria function in SAMP8 mice

Abstract

Abstract: AIM: To investigate the protective effects of icariin (ICA) on cerebral mitochondria in senescence accelerated mice (SAM).METHODS: The 8-month-old senescence-accelerated mouse prone /8(SAMP8) mice were divided into SAMP8 group, Donepezil Hydrochloride (DON)-treated SAMP8 group (1 mg/kg), Piracetam (PIR)-treated SAMP8 group (200 mg/kg) and ICA-treated SAMP8 groups (75 mg/kg and 150 mg/kg). The age-matched senescence-accelerated mouse resistance 1( SAMR1) mice served as control. After treatment for 30 d, the cerebral mitochondria were isolated. The ATP production, reactive oxygen species (ROS) level, oxidative phosphorylation parameters and mitochondrial permeability transition pore (MPTP) were detected to evaluate the function of mitochondria in SAM mice.RESULTS: Respiratory control index, ADP/O ratio, State Ⅲ respiration and oxidative phosphorylation rate (OPR) were significantly lower in SAMP8 than those in SAMR1(P<0.05). State Ⅳ respiration did not significantly change in SAMP8 compared with that in SAMR1. At the same time, the loss of mitochondrial membrane potential(MMP), the decreased of ATP content, the increase of mitochondrial swelling degree and the increased level of ROS were also observed in SAMP8 brain mitochondria. ICA at the dosage of 75 mg/kg and 150 mg/kg significantly improved oxidative phosphorylation process, mitochondria swelling degree, MMP and other biochemical functions.CONCLUSION: ICA has a protective effect on cerebral mitochondrial impairment in SAMP8 mice.

Key words: Icariin, Senescence accelerated mice, Alzheimer's disease, Mitochondria

CLC Number:

R965.2

WU Man-gang, HE Xiao-li, LIU Dong-hua, QIANG Gui-fen, SHI Li-li, LI Xiao-xiu, ZU Mian, BI Ming-gang, DU Guan-hua. Protective effects of icariin on cerebral mitochondria function in SAMP8 mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 721-726.

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