Reward alteration after cocaine abstinent and orexin expression in lateral hypothalamus

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2010, Vol. 15 ›› Issue (9): 986-990.

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Reward alteration after cocaine abstinent and orexin expression in lateral hypothalamus

LIU Sheng¹, ZHOU Wen-hua¹, GARY Aston-jonesi²

¹Ningbo Addiction Research and Treatment Center, Ningbo 315010,Zhejiang, China;
²Medical University of South Carolina, Charleston, SC 29425, USA

Received:2010-07-13 Revised:2010-09-10 Online:2010-09-26 Published:2020-09-17

Abstract

Abstract: AIM: To investigate the role of lateral hypothalamus orexin neurons in the rewarding properties of cocaine during chronic cocaine abstinence.METHODS: Rats were initially treated chronically with daily injections of cocaine (10 days of escalating doses of cocaine, 10-30 mg/kg, i.p.). Following 2 weeks of forced abstinence from cocaine, rats were subjected to a conditioned place preference (CPP)procedure for cocaine. After CPP test, rats were received Fos/Orexin double immunohistochemistry in the lateral hypothalamus.RESULTS: Compared with saline-pretreated group,cocaine preference in abstinent rats was significantly elevated [abstinent rats vs saline rats: (250±24)s vs (132±44)s, cocaine-paired side minus saline-paired side, P<0.01];the number of Fos-positive neurons (±SEM) in the lateral hypothalamus in cocaine-treated rats was significantly elevated [cocaine rats vs saline rats: (41±4 ) vs (16±4), P<0.05]; animals that were pretreated with cocaine had a significantly greater percentage of orexin neurons that were Fos-positive [cocaine rats vs saline rats: (39.4±2.4)% vs (22.5±3.2)%, P<0.01]. A significant correlation was found between the preference scores and the percentage of orexin neurons that were Fos activated (R=0.66,P<0.05).CONCLUSION: Lateral hypothalamus orexin neurons maybe play an important role in the rewarding properties of cocaine during chronic cocaine abstinence.

Key words: Reward, Orexin, Addiction, Lateral hypothalamus

CLC Number:

R965.2

LIU Sheng, ZHOU Wen-hua, GARY Aston-jonesi. Reward alteration after cocaine abstinent and orexin expression in lateral hypothalamus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(9): 986-990.

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Reward alteration after cocaine abstinent and orexin expression in lateral hypothalamus

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