Effects of di (2-ethylhexyl) phthalate on the prostaglandin E-2 levels and cyclooxygenase-2 mRNA expression of testicular tissue in adult rats

Abstract

Abstract: AIM: To explore the effects on prostaglandin E-2 and cyclooxygenase-2 mRNA expression of testicular spermatogenic cells in adult rats exposed to di (2-ethylhexyl) phthalate (DEHP). METHODS: 40 male Sprague-Dauley rats were divided into four groups in random: normal control group and 3 groups exposed to DEHP at the dose of 100,200 or 500 mg/kg bodyweight per day by gavage respectively. One month later,the rats were sacrificed. The expression of testicular prostaglandin E-2 was measured by ELISA. The expression level and location of cyclooxygenase-2 and cyclooxygenase-2 mRNA in testicular tissue were detected by immunohistochemistry and RT-PCR, respectively.RESULTS: The expression levels of prostaglandin E-2 and cyclooxygenase-2 of testicular tissue decreased in 3 groups compared with normal control group,cyclooxygenase-2 was mainly expressed in spermatogonia. CONCLUSION: DEHP can damage spermatogenic cells of adult mice,the down-regulating expression of cyclooxygenase-2 mRNA may be involved in it.

Key words: Di (2-ethylhexyl) phthalate, Testis, Prostaglandin E-2, Cyclooxygenase-2 mRNA , , , ,

CLC Number:

R965.2

ZHANG Hao-chuan,CHEN Cong-de,BAO Xiao-zhou,CHEN Xiao-ming. Effects of di (2-ethylhexyl) phthalate on the prostaglandin E-2 levels and cyclooxygenase-2 mRNA expression of testicular tissue in adult rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(1): 38-41.

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