A better method to test bacterial endotoxin in lentinan for injection with the specific Tachypleus Amebocyte Lysate (B,C factor)

Abstract

Abstract: AIM: To study the effect of the method to test bacterial endotoxin in lentinan for injection with the specific tachypleus amebocyte lysate (B,C factor).METHODS: According to the bacterial endotoxin test approved by Chinese phamocopoeia 2005 edition, to test bacterial endotoxin in lentinan for injection with the specific tachypleus amebocyte lysate (B,C factor) and with tachypleus amebocyte lysate respectively.RESULTS: It was accurate, sensitive, and there was no false positive reaction to test bacterial endotoxin in lentinan for injection with the specific tachypleus amebocyte lysate (B,C factor).CONCLUSION: It is a better method with the specific tachypleus amebocyte lysate (B,C factor) to test bacterial endotoxin in lentinan for injection.

Key words: lentinan for Injection, bacterial endotoxin test, Specific Tachypleus Amebocyte Lysate (B,C factor)

CLC Number:

R965.1

YAO Xin-bao, TIAN Xiu-li, WEI Guo-yi. A better method to test bacterial endotoxin in lentinan for injection with the specific Tachypleus Amebocyte Lysate (B,C factor)[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(11): 1257-1259.

References

[1] 朱珍梅, 周菊芬. 新一代弃G因子鲎试剂[J]. 中国冶金工业医学杂志, 1999, 16(5): 315.
[2] 吴伟洪, 罗希远. 一种更敏感专一的重新组合鲎凝固酶原、BC因子的试剂(简称鲎BC因子试剂)的制备和测定[J]. 现代应用药学, 1987, 4(6): 864.
[3] 郑丽莉. 香菇多糖氯化钠注射液细菌内毒素检查方法建立及方法学研究[J]. 中国药品标准, 2007, 8(6): 54-56.
[4] 林小婵, 黄敬荣. 香菇多糖氯化钠注射液细菌内毒素检测方法的试验研究[J]. 海峡药学, 2008, 20(4): 49-50.
[5] 国家药典委员会. 中国药典[M]. 北京: 化学工业出版社, 2005版, 二部, 附录85-88.
[6] 程义琳, 吴蕙玲. 鲎试剂灵敏度对检测结果的影响[J]. 中国药师, 2005, 8(4): 344-345.
[7] 张红霞, 陈慧, 马金刚. 影响鲎试剂灵敏度测定结果因素的分析[J]. 中国药师, 2007, 10(8): 825-826.
[8] Kakinuma A, Asano T, Torii H, et al. Gelation of Limulus amoebocyte lysate by an antitumor (1 leads to3)-beta-D-glucan[J]. Biochem Biophys Res Commun, 1981, 101(2): 434-439.
[9] 张永坚, 冯聚锦. β-葡聚糖与内毒素共同作用对细菌内毒素检查的影响[J]. 中国药品标准, 2002, 3(6): 16-19.
[10] 叶良君, 张强, 张涛, 等. β-葡聚糖对含糖大输液细菌内毒素检查的影响[J]. 中国药业, 2008, 17(19): 24-25.
[11] 钟劲松, 冯聚锦. 葡萄糖、氯化钠注射液细菌内毒素检查假阳性原因的探讨[J]. 中国药学杂志, 2002, 37(6): 449-451.
[12] 施震, 尹银嘉, 李华. 特异性鲎试剂和非特异性鲎试剂对艾迪注射液中细菌内毒素检测的比较[J]. 中国生化药物杂志, 2003, 24(3): 137-138.
[13] 尹银嘉, 商小曼. 特异性鲎试剂检测人参多糖注射液中的细菌内毒素[J]. 中国医院药学杂志, 2002, 22(11): 677-679.
[14] 张慧莉. 四种大输液的细菌内毒素检查[J]. 中国药品标准, 2003, 4(2): 28-30.
[15] 唐坤. 弃G因子鲎试剂检测在大输液中应用[J]. 华夏医学, 2000, 13(2): 219-221.
[16] 张贵宝, 徐紫娟, 白文东. 鲎试剂用于20%甘露醇注射液细菌内毒素检测[J]. 工企医刊, 2007, 20(6): 18-20.