Hyperpolarization of hydrogen sulfide on the membrane potential of vascular smooth muscle cell in rat middle cerebral artery

Abstract

Abstract: AIM: To study the hyperpolarizing effect of hydrogen sulfide (H₂S) and endothelium-derived hyperpolarizing factor (EDHF) on the resting membrane potential of vascular smooth muscle cell (VSMC) in rat middle cerebral artery (MCA).METHODS: By using the record of resting membrane potential of VSMC in the isolated rat MCAs, the hyperpolarizing effects of acetylcholine (ACh), NaHS were detected, respectively.RESULTS:In rat MCAs, 10^-7-10^-4.5 mol/L ACh had significant and concentration-dependent hyperpolarizing effect on the resting membrane potential of VSMC in the absence and presence of 3×10^-5 mol/L Nω-nitro-L-arginine-methyl-ester (L-NAME, an inhibitor of nitric oxide synthase) and 1×10^-5 mol/L indomethacin (Indo, an inhibitor of PGI₂ synthesis). The hyperpolarizing effect of ACh in the presence of L-NAME and Indo was almost abolished by tetraethylammonium (TEA, an inhibitor of calcium-activated K⁺ channels at 1×10^-3 mol/L) or 1×10^-4 mol/L DL-propargylglycine (PPG), an inhibitor of endogenous H₂S synthase (cystathionine-γ-lyase, CSE). NaHS, a donor of H₂S had the concentration-dependent hyperpolarizing effect on the resting membrane potential of VSMC in rat MCAs, and the hyperpolarization was markedly inhibited by 1×10^-3 mol/L TEA.CONCLUSION: H₂S might mediate the non-NO- non-PGI₂ hyperpolarization, the so-called EDHF response on the resting membrane potential of VSMC in rat MCAs.

Key words: Endothelium-dependent hyperpolarizing factor, Hydrogen sulfide, Rat middle cerebral artery, Vascular smooth muscle cell, Resting membrane potential, Hyperpolarization

CLC Number:

R965.2

CAI Sheng-nian, FAN Yi-fei, CHEN Zhi-wu. Hyperpolarization of hydrogen sulfide on the membrane potential of vascular smooth muscle cell in rat middle cerebral artery[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 155-159.

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