Quantitative analysis of circulating DNA in plasma of lung cancer patients with metastasis or recurrence

Abstract

Abstract: AIM: To quantify the circulating DNA in plasma from patients with lung cancer and investigate the relationship with the circulating DNA and neoplasm metastasis or recurrence in lung cancer patients.METHODS: Blood samples were collected from 98 lung cancer patients,44 patients with non-neoplastic diseases,and 60 healthy volunteers. The circulating DNA was extracted and analyzed by fluorescent dye(SYBR green I)staining.RESULTS:The circulating DNA concentration in the lung cancer, was significantly higher than which patients with non-neoplastic diseases and the healthy volunteers.The level of circulating DNA was higher in lung caner patients with metastasis or recurrence.CONCLUSION: Circulating DNA in plasma may become a new tumor marker to monitor metastasis or recurrence of lung cancer.

Key words: Lung cancer, Circulating DNA, Metastasis, Recurrence

CLC Number:

R734

LIU Hong-jie, YE Bin, CHENG Jing, JI Zhao-ning. Quantitative analysis of circulating DNA in plasma of lung cancer patients with metastasis or recurrence[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(2): 209-213.

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